Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1992-01-21
1992-10-20
Lacey, David L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352401, 4352402, 43524027, 4352403, 43524031, 530350, 5303881, 53038815, 530865, C12N 1500, C12N 500, C12N 502, C07K 300
Patent
active
051569647
ABSTRACT:
This invention is in the field of cell and/or tissue culture. In particular, this invention relates to methods which adapt cells to a desired phenotype by exposing the cells to high levels of ammonia in culture, and subsequently transferring the adapted cells to a new culture medium in which there is no initial level of ammonia or the initial level of ammonia is below the level to which cells have been exposed to during the adaptation process. In this new culture medium, the adapted cells express the desired phenotype of growing to a higher viable cell density, and/or remaining viable for a longer period of time, and/or producing more of a desired cell product than their non-adapted counterparts grown in the same medium. This invention includes the adapted cells produced thereby and their cell products.
REFERENCES:
patent: 4724206 (1988-02-01), Rupp et al.
Beeraj R. D. Can. J. Microbiol. 29:563-569, 1983.
Miller et al. Bioprocess Engineering, 3:3 pp. 113-122, 1988.
Pedersen et al. Annals of the New York Academy of Sciences vol. 506, Biochemical Engineering V, 1987.
Inlow Duane
Maiorella Brian
Shauger Andrea E.
Cetus Corporation
Giotta Gregory J.
Heslin James M.
Lacey David L.
Wang Gian P.
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