Methods for activating natural killer (NK) cells and means...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Two or more cell types – per se – in co-culture

Reexamination Certificate

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C435S325000, C435S372000, C435S373000, C435S377000, C435S006120, C435S007240, C424S093100, C424S093300, C424S093700, C424S192100

Reexamination Certificate

active

06849452

ABSTRACT:
The present invention relates to a process for activating natural killer cells comprising bringing NK cells into contact with dendritic cells in vitro, ex vivo or in vivo. The invention also relates to cell compositions comprising activated NK cells, NK cell-dendritic cell co-cultures or dendritic cells, and to their use to stimulate the cytolytic activity of NK cells or natural immunity in vivo. The invention also relates to a NK cell stimulation factor present in the dendritic cell membrane, and to triggering media and factor(s) for dendritic cells and to their use, either alone or in combination, to stimulate NK activity, in particular in vivo. The invention can be used to control NK cell activity in vitro, ex vivo or in vivo, in particular under pathological conditions.

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Rabinowich et al. (1992) J. Immunol., vol. 149, 340-349.*
Hsu et al. (1996) Nat. Med., vol. 2(1), 52-58.*
Shah et al., Dendritic cells that have interacted with antigen are targets for natural killer cells, 1985, J. Exp. Med., vol. 162, pp. 625-636.
Leibson, Signal Transduction during Natural Killer Cell Activation: Inside the Mind of a Killer, Jun. 1997, vol. 6 pp. 655-661.*
Gorak et al., “Dendritic cells, but not macrophages, produce IL-12 immediately followingLeishmania donovani nfection,” Eur. J. Immunol. 28: 687-695 (1998).

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