Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2011-06-28
2011-06-28
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S025000
Reexamination Certificate
active
07968314
ABSTRACT:
The invention provides a method of evaluating metabolism-based drug interactions. The method involves selecting time points for the determination of the inactivation rate constant of a time-dependent enzyme inhibitor based on the results of a multi-time point IC50 test. Advantageously, with the subject invention, the determination and use of the multi-time point IC50 test provides an indication of the inactivation rate of a test compound and eliminates trial and error tests associated with the selection of appropriate assay conditions for the second assay conducted to determine the inactivation rate constant of the test compound.
REFERENCES:
patent: 7608616 (2009-10-01), Cartt
Perloff E. et al. Validation of Cytochrome P450 Time Dependent Inhibition Assays. Xenobiotica 2009 39(2)99-112.
Ghanbari, F., Rowland-Yeo, K., Bloomer, J.C., Clarke S.E., Lennard, M.S., Tucker, G.T., and Rostami-Hodjegan, A., “A Critical Evaluation of the Experimental Design of Studies of Mechanism Based Enzyme Inhibition, With Implications for In Vitro-In Vivo Extrapolation,” Curr. Drug Metab., Apr. 2006; 7(3):315-34.
Obach, R. S., Walsky, R. L., and Venkatakrishnan, K., “Mechanism-Based Inactivation of Human Cytochrome P450 Enzymes and the Prediction of Drug-Drug Interactions,” Drug Metab. Dispos., Feb. 2007; 35(2):246-55, Epub Nov. 8, 2006.
Becton Dickinson and Company
Gitomer Ralph
Hoffmann & Baron , LLP
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