Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Reexamination Certificate
2005-11-29
2005-11-29
Chin, Christopher L. (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
C422S068100, C435S006120, C435S007100, C435S007900, C435S174000, C435S287100, C436S524000, C436S528000, C436S530000, C436S531000, C436S503000
Reexamination Certificate
active
06969615
ABSTRACT:
The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.
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Baibakova Galina
Emmert-Buck Michael R.
Gardner Kevin
Hartmann Dan-Paul
Hewitt Stephen M.
20/20 GeneSystems, Inc.
Chin Christopher L.
Klarquist & Sparkman, LLP
The United States of America as represented by the Department of
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