Methods, compositions, and cells for encapsidating...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S091100, C435S091330, C435S091400, C435S094000, C435S091420, C530S300000, C514S024000

Reexamination Certificate

active

06893865

ABSTRACT:
Isolated recombinant polynucleotides comprising elements which promote encapsidation into AAV particles, packaging cells comprising the recombinant polynucleotides, and methods for their use are provided in the present invention. These isolated recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) operably linked to one or more heterologous genes to be encapsidated. The constructs may be either integrated into a mammalian cell genome, maintained episomally, or provided transiently.

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