Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-05-18
2000-05-23
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435 9121, 435 9151, 536221, 536231, 536 243, 536 2532, 536 237, 935 77, C12Q 168
Patent
active
060664584
ABSTRACT:
Methods for determining quantities of nucleic acid sequences in samples undergoing amplification utilize amplification ratio estimates (R*) in operations to accurately perform absolute quantitation even when amplification factors for the target and control sequences undergoing amplification are different, time dependent or vary as a function of starting concentrations of nucleic acid sequences. These operations also take into account conversion efficiencies associated with the conversion of probes upon generation of target or control amplicons, but do not require the explicit calculation of such efficiencies. The operations also recognize that a preferred R* should be determined based on a preferred statistical criterion to improve quantitation. In addition, the use of standard samples having known starting concentrations of target and control sequences therein may enable accurate absolute quantitation without the explicit calculation of amplification ratio estimates.
REFERENCES:
patent: 5270184 (1993-12-01), Walker et al.
patent: 5455166 (1995-10-01), Walker
patent: 5457027 (1995-10-01), Nadeau et al.
patent: 5547861 (1996-08-01), Nadeau et al.
patent: 5550025 (1996-08-01), Walker
patent: 5593867 (1997-01-01), Walker et al.
patent: 5710029 (1998-01-01), Ryder et al.
patent: 5736333 (1998-04-01), Livak et al.
patent: 5863736 (1999-01-01), Haaland et al.
Gibson et al.; "A Method for Real Time Quantitative RT-PCR"; Genome Research, 6:995-1001 (1996).
Heid et al.; "Real Time Quantitative PCR"; Genome Research, 6:986-994 (1996).
A. Rashtchian; "Amplification of RNA"; PCR Methods and Applications, 4:S83-S91 (1994).
Rodriquez et al.; "A Novel Method for the Isolation of Tissue-Specific Genes"; Nucleic Acids Res., 20(13):3528 (1992).
Edmands et al.; "Rapid RT-PCR Amplification from Limited Cell Numbers"; PCR Methods and Applications, 3:17-319 (1994).
McCulloch et al.; "An Evaluation of Competitor Type and Size for Use in the Determination of mRNA by Competitive PCR"; PCR Methods and Applications, 4:219-226 (1995).
Clementi et al.; "Quantitative PCR and RT-PCR in Virology"; PCR Methods and Applications, 2:191-196 (1993).
F. Ferre; "Quantitative or Semi-Quantitative PCR: Reality Versus Myth"; PCR Methods and Applications, 2:1-9 (1992).
M. Piatak, Jr., et al., "Research Report. Quantitative Competitive Polymerase Chain Reaction for Accurate Quantitation of HIV DNA and RNA Species," BioTechniques 14(1), pp. 70-80 (1993).
G. Haberhausen et al., "Comparative Study of Different Standardization Concepts in Quantitative Competitive Reverse Transcription-PCR Assays," Journal of Clinical Microbiology 36(3), pp. 628-633 (Mar. 1998).
L. Raeymaekers, "Quantitative PCR: Theoretical Considerations with Practical Implications," Analytical Biochemistry 214, pp. 582-585 (1993).
L. Raeymaekers, "A Commentary on the Practical Applications of Competitive PCR," Genome Research 5, pp. 91-94 (1995).
Tan et al.; "PAF and TNF Increase the Precursor of NF-kappa B p50 mRNA in Mouse Intestine: Quantitative Analysis by Competitive PCR"; Biochimica et Biophysica Acta, 1215:157-162 (1994).
Kellogg et al.; "Quantitation of HIV-1 Proviral DNA Relative to Cellular DNA by the Polymerase Chain Reaction"; Analytical Biochemistry, 189:202-208 (1990).
Holland et al.; "Detection of specific polymerase chain reaction product by utilizing the 5' .fwdarw. 3' exonuclease activity of thermus aquaticus DNA polymerase"; Proc. Natl. Acad. Sci. USA, 88:7276-7280 (1991).
Walker et al.; "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system"; Proc. Natl. Acad. Sci. USA, 89:392-296 (1992).
Furtado et al.; "Changes in the Viral mRNA Expression Pattern Correlate with a Rapid Rate of CD4.sub.+ T-Cell Number Decline in Human Immunodeficiency Virus Type 1-Infected Individuals"; J. of Virology, 69(4):2092-2100 (1995).
Kwoh et al.; "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format"; Proc. Natl. Acad. Sci. USA, 86:1173-1177 (1989).
Pang et al.; "High levels of unintegrated HIV-1 DNA in brain tissue of AIDS dementia patients"; Nature, 343:85-89 (1990).
Lizardi et al.; "Experonential Amplification of Recombinant-RNA Hybridization Probes"; Bio/Technology, 6:1197-1202 (1988).
Slamon et al.; "Human Breast Cancer: Correlation of Relapse and Survival with Amplification of the HER-2
eu Oncogene"; Science, 235:177-182 (1987).
Sooknanan et al.; "NASBA: A Detection and Amplification System Uniquely Suited for RNA"; Bio/Technology, 13:563-564 (1995).
Piatak et al.; "High Levels of HIV-1 ijn Plasma During All Stages of Infection Determined by Competitive PCR"; Science, 259:1749-1754 (1993).
Dean Cheryl H.
Haaland Perry D.
Nadeau James G.
Nycz Colleen M.
Spargo Catherine A.
Becton Dickinson and Company
Fugit Donna R.
Horlick Kenneth R.
Taylor Janell E.
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