Methods and vectors for selectively cloning exons

Details Methods and vectors for selectively cloning exons Methods and vectors for selectively cloning exons

1991-07-03

1993-10-12

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 4352402, 536 231, C12N 1510, C12N 1585, C12N 510, C07H 2104

Patent

active

052524752

ABSTRACT:
The invention relates to a method for selectively cloning exons in which an ukaryotic genomic DNA fragment to be assayed is cloned into a shuttle vector in a cloning site of an intron flanked by exons. Upon expression in eukaryotic cells, exons present in the cloned DNA are spliced to the exons flanking the intron. After amplification of the cDNA of the expressed mRNA with primers specific for the exons in the vector by PCR, selectively cloned exons can be detected due to the size of the PCR product. The invention also relates to vectors used to carry out the method of the invention as well as to exon libraries.

REFERENCES:
Cullen, Cell, vol. 46, 973-982 (1986).
Auch et al. (Dec. 1990), Nucl. Acids Res., vol. 18 (22), pp. 6743-6744.
Cepko et al. (Jul. 1984), Cell, vol. 37, pp. 1053-1062.

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