Methods and vector constructs useful for production of...

Details Methods and vector constructs useful for production of... Methods and vector constructs useful for production of...

2005-09-13

2005-09-13

Mosher, Mary E. (Department: 1648)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S369000, C435S235100, C435S239000

Reexamination Certificate

active

06943019

ABSTRACT:
Methods for efficient production of recombinant AAV employ a host cell containing a first nucleic acid molecule comprising from 5′ to 3′, a parvovirus P5 promoter, a spacer, an AAV rep sequence and an AAV cap gene sequence, wherein said spacer is of sufficient size to reduce expression of the rep78 and rep68 gene products; a second nucleic acid molecule comprising a minigene comprising a transgene flanked by AAV inverse terminal repeats (ITRs) and under the control of regulatory sequences directing expression thereof in a host cell; and helper functions essential to the replication and packaging of rAAV, which functions are not provided by the first or second nucleic acid molecules. Host cells and molecule constructs are also described.

REFERENCES:
patent: 5622856 (1997-04-01), Natsoulis
patent: 5658776 (1997-08-01), Flotte et al.
patent: 6027931 (2000-02-01), Natsoulis et al.
patent: 6482634 (2002-11-01), Wilson et al.
patent: WO-95/06743 (1995-03-01), None
patent: WO 96/17947 (1996-06-01), None
patent: WO-96/40240 (1996-12-01), None
patent: WO 96/40955 (1996-12-01), None
patent: WO 97/06272 (1997-02-01), None
patent: WO 98/10086 (1998-03-01), None
patent: WO 98/46728 (1998-10-01), None
patent: WO 99/07833 (1999-02-01), None
patent: WO 99/15677 (1999-04-01), None
patent: WO 99/15685 (1999-04-01), None
Li et al. Journal of Virology 71(7):5236-5243, Jul. 1997.
B.J. Carter in “Handbook of Parvoviruses”, ed., P.Tijssen, CRC Press, pp. 155-168 (1990), month not available.
R. Kotin, “Prospects for the Use of Adeno-Associated Virus as a Vector for Human Gene Therapy”,Hum. Gene Ther., 5:793-801 (Jul. 1994).
J.D. Watson et al., Molecular Biology of the Gene, The Benjamin/Cummings Publishing Company, Inc., 1987, XP002091111, pp. 703-705, month not available.
J. Sambrook et al., “Molecular Cloning. A Laboratory Manual”, Cold Spring Harbour Laboratory Press, 1989, XP002091112, pp. 16.5-16.6, month not available.

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