Methods and transgenic mouse model for identifying and...

Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal

Reexamination Certificate

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C800S003000, C800S008000, C800S009000, C435S325000

Reexamination Certificate

active

07119249

ABSTRACT:
The present invention provides a transgenic non-human animal, in particular a transgenic mouse encoding the NOX and Duox family of proteins, which generate reactive oxygen intermediates (ROI). The present invention additionally comprises cells and cell lines containing transgenes encoding for members of the NOX and Duox family of proteins. The present invention further comprises methods and compositions for evaluating regulators of abnormal cell growth and in the development of compounds that effect ROI expression.

REFERENCES:
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Capecchi MR, “The new mouse genetics: altering the genome by gene targeting,” Trends in Genetics 5(3): 70-76, 1989.
Suh et al., “Cell transformation by the superoxide-generating oxidase Mox1,” Nature 401:79-82, 1999.
Cowan et al, “Targeting gene expression to endothelium in transgenic animals: a comparison of the human ICAM-2, PECAM-1 and endoglin promoters,” Xenotransplanation 10:223-231, 2003.
Hammer et al., “Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human B2m: an animal model of HLA-B27-associated human disorders,” Cell 63:1099-1112, 1990.□□
Cheng et al., “Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, Nox5,” Gene 269: 131-140, 2001.
Edens et al., “Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a multidomain oxidase/peroxidase with homology to the phagocyte oxidase subunit gp91phox,” Journal of Cell Biology 154(4): 879-891, 2001.
Weber et al., “The activity of highly promiscuous AP-1 element can be confined to neurons by a tissue-selective repressive element,” Journal of Neuroscience 18(14): 5264-5274, 1998.

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