Methods and transformed mammalian lymphocyte cells for producing

Details Methods and transformed mammalian lymphocyte cells for producing Methods and transformed mammalian lymphocyte cells for producing

1994-06-27

1998-09-15

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435326, 5303871, 5303873, 536 2353, C12N 1500, C12N 1513, C07K 1600

Patent

active

058077154

ABSTRACT:
Methods for producing functional immunoglobulin are provided. The methods involve transfecting and expressing exogenous DNA coding for the heavy and light chains of immunoglobulin. In some embodiments, chimeric immunoglobulins are provided having variable regions from one species and constant regions from another species by linking DNA sequences encoding for the variable regions of the light and heavy chains from one species to the constant regions of the light and heavy chains respectively from a different species. Introduction of the resulting genes into mammalian host cells under conditions for expression provides for production of chimeric immunoglobulins having the specificity of the variable region derived from a first species and the physiological functions of the constant region from a different species.

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