Methods and systems for removing autofluorescence from images

Details Methods and systems for removing autofluorescence from images Methods and systems for removing autofluorescence from images

2007-11-30

2011-10-04

Perungavoor, Sath V (Department: 2624)

Image analysis

Applications

Biomedical applications

C382S294000, C702S019000

Reexamination Certificate

active

08031924

ABSTRACT:
Method and systems for removing any inherent autofluorescence associated with a biological material, comprising: acquiring a first reference image of the biological material; acquiring a first set of one or more images of the biological material using one or more filters corresponding to one or more information dyes; applying the one or more additional dyes to the biological material and then acquiring a second set of one or more images comprising a separate image of the biological material with each of the filters corresponding to the information dyes and a second reference image of the biological material; coregistering the first and second reference images; and then removing any inherent autofluorescence exhibited in the informational images acquired.

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