Methods and systems for producing HIV antigens

Details Methods and systems for producing HIV antigens Methods and systems for producing HIV antigens

1989-04-26

1993-04-20

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 5, 435 691, 435 693, 435 697, 4351723, 43525233, 4353201, 435974, 536 2372, 930221, 935 23, 935 27, 935 38, 935 45, 935 47, 935 72, 935 73, C12N 121, C12N 1549, C12N 1562, C12N 1570

Patent

active

052042596

ABSTRACT:
The present invention relates to a DNA segment encoding a recombinant HIV p24 protein or HIV p24-gp41 fusion protein and a recombinant DNA (rDNA) molecule capable of expressing either protein. Cells transformed with the rDNA, methods for producing the fusion protein and diagnostic methods and systems using the fusion protein are also described.

REFERENCES:
patent: 4808536 (1989-02-01), Chang et al.
patent: 4925784 (1990-05-01), Crowl et al.
J. G. Huisman et al. (1987) Vox Sanguinis 53:31-36.
P. Tijssen, "Practices and Theory of Enzyme Immunoassays", in Laboratory Techniques in Biochemistry and Molecular Biology, vol. 15, R. H. Burdon et al., ed. Elsevier, Amsterdam (1985), pp. 14-17, 470-471, 482-483.
N. T. Chang et al. (1985) Science 228: 93-96.
M. Guyader et al. (1987) Nature 326: 662-669.
Shoeman et al., Analytical Biochemistry, 161:370-379 (1987).
Ratner et al., Nature, 313:277-285 (1985).
Dowbenko et al., Proc. Natl. Acad. Sci., U.S.A., 82:7748-7752 (1985).
Ghrayeb et al., DNA, 5(2):93-99 (1986).

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