Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Reexamination Certificate
2011-05-17
2011-05-17
Therkorn, Ernest G (Department: 1776)
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
C210S656000, C210S198200, C210S502100, C530S413000, C530S417000
Reexamination Certificate
active
07943046
ABSTRACT:
Embodiments of the present invention provide a method of chromatographic delipidation comprising separating a lipid-containing sample on a superficially porous stationary phase at greater than about 70° C., at least about 80° C., having at least one mobile phase comprising an ion-pairing agent in water, an ion-pairing agent in an organic modifier, an acid in an organic modifier, and an alcohol. The invention provides minimal protein losses and high run-to-run reproducibility. The on-column delipidation method aventageously utilize reversed phase liquid chromatography.
REFERENCES:
patent: 3505785 (1970-04-01), Kirkland
patent: 4070283 (1978-01-01), Kirkland
patent: 4477492 (1984-10-01), Bergna et al.
patent: 4847159 (1989-07-01), Glajch et al.
patent: 4920152 (1990-04-01), Regnier et al.
patent: 4929700 (1990-05-01), Halenbeck et al.
patent: 5108595 (1992-04-01), Kirkland et al.
patent: 5439829 (1995-08-01), Anderson et al.
patent: 5731166 (1998-03-01), Geczy et al.
patent: 5885921 (1999-03-01), Krupey
patent: 5990284 (1999-11-01), Mahiou et al.
patent: 6057468 (2000-05-01), Kirkland et al.
patent: 6071705 (2000-06-01), Wands et al.
patent: 6265542 (2001-07-01), Fahrner et al.
patent: 6538126 (2003-03-01), Cho et al.
patent: 6730228 (2004-05-01), Petro et al.
patent: 6829539 (2004-12-01), Goodlett et al.
patent: 6866782 (2005-03-01), Scapol et al.
patent: 7323553 (2008-01-01), Fahrner et al.
patent: 7396468 (2008-07-01), Boyes et al.
patent: 7449116 (2008-11-01), Martosella et al.
patent: 2002/0168682 (2002-11-01), Goodlett et al.
patent: 2003/0027354 (2003-02-01), Geli
patent: 2004/0115725 (2004-06-01), Pieper et al.
patent: 2004/0200776 (2004-10-01), Ivanov et al.
patent: 2006/0128665 (2006-06-01), Leigh et al.
patent: 02/055654 (2002-07-01), None
patent: 2004/035169 (2004-04-01), None
Bidlingmeyer, B. et al., “High Velocity Chromatography of Biomacromolecules”, Copyright 2001.
Chernokalskaya, E. et al., “Ultrafiltration for proteomic sample separation”, Millpore Corporation, Life Science Division, Danvers , MA USA, Electrophoresis 2004, vol. 25, pp. 2461-2468.
Eichacker, L.A. et al., Hiding behind Hydrophobicity Department fur Biologie I,.Ludwig=Masimilians Universitat Muchen, Muchen, Germany and Hoffmann-La Roche, Roche Centre for Medical Geomics, Basel, Switzerland, Journal of Biological Chemistry © 2004 by the American Society of Biochemistry and Molecular Biology, Inc., vol. 279, No. 49, Issue of Dec. 3, pp. 50915-50922, 2004.
Elortza, F. et al., “Proteomic Analysis of Glycvosylphosphatidylinositol-anchored Membrane Proteins”, The American Society for Biochemistry and Molecular Biology, Inc. © 2003; Molecular & Cellular Proteomics, vol. 2, No. 12, pp. 1261-1270.
Girogion Righetti, P. et al., “Prefractionation techniques in proteoma analysis”, Proteomics, 2003, vol. 3, pp. 1397-1407.
Han, J. et al., Proteolysis and Mass Spectrometic Analysis of an Integral Membrane: Aquaporin O, Department of Cell and Molecular Pharmacology, Medical Univerisity of South Carolina, Charleston, SC, Journal of Proteome Research, 2004, vol. 3, pp. 807-812, © 2004 American Chemical Society, published on the web May 19, 2004.
Lescuyer, P. et al., “Comprehensive proteome analysis by chromatographic protein prefractionation”, Electrophoresis, 2004, vol. 25, No. 7-8, pp. 1125-1135, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Liu, H. et al., “A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics”, Analytical Chemistry, vol. 76, No. 14, Jul. 15, 2004, pp. 4193-4201.
Martosella, J. et al., “Optimization of Reversed-Phase Separations of Human Serum Proteins and Use in Protein Identification Human Serum Proteins and Use in Protein Identification”, Agilent Technologies, Wilmington, DE 19808; ASMS 2004, ThPF483.
Martosella, J. et al., “Reversed-Phase High-Performance Liquid Chromatographic Prefractionation of Immunidepleted Human Serum Proteins to Enhance Mass Spectrometry Identification of Lower-Abundant Proteins”, Agilent Technologies, Wilmington, DE 19808, Journal of Proteome Research 2005, No. 4, pp. 1522-1537, published Aug. 4, 2005.
Neverova, I, et al., “Application of reversed phase high performance liquid chromatography for subproteomic analysis of cardiac muscle”, Proteomics, 2002, vol. 2, No. 1, pp. 22-31, WILEY-VCH Verland GmbH, 69451 Weinheim, 2002.
Nuhse, T. et al., Large-scale Analysis of in Vivo Phosphorylated Membrane Proteins by Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry, © 2003 by The American Society for Biochemistry and Molecular Biology, Inc., Molecular & Cellular Proteomics, vol. 2, No. 11, pp. 1234-1243.
Poster, P. 1020, HPLC 2004, Philadelphia, PA, Wed-Jun. 16, 2004, “Reversed-phase high-performance liquid chromatography prefractionation prior to two-dimensional difference gel electrophoresis and mass spectrometry identifies new differentially expressed proteins, between striate cortex of kitten and adult cat”, Electrophoesis 2003, vol. 24, pp. 1471-1481, 2003, WILEY-VCH Verland GmbH, 69451 Weinheim.
Ricker, R. et al., “A family of superficially porous particle HPLC columns for versatility in the rapid analysis of proteins and polypeptides at low and high pH”, HPLC 2004, Philadelphia, PA, Wed-Jun. 16, 2004, Poster P-1020.
Ricker, R. et al., “High velocity chromatography A new technique for macromolecule separation”, Life Science and Chemical Analysis Group, Talk #763, Presented at Pittcon, Mar. 17-21, 2002, New Orleans, LA, USA.
Ricker, R., Comparison of ZORBAX Poroshell 300Extend-C18 and Totally Porous Packing in Achieving Very Rapid, High-pH Separation of Peptides, Biotechnology/QA/QC/Basic R&D, Agilent Technologies, Inc., 2004, Printed in the USA, Feb. 20, 2004.
Woodward, C. et al., “Fast Separation of Large and Heterogeneous Proteins Using ZORBAX Poroshell C18, C8 and C3 Phases”, Agilent Technologies, Inc., 2003, Printed in USA, Oct. 17, 2003.
Hage, D., “Affinity Chromatography: A Review of Clinical Applications,” Clinical Chemistry 45:5, pp. 593-615 (1999).
Hage, D. , “Survey of recent advances in analytical applications of immunoaffinity chromatography,” Journal of Chromatography B, 715, pp. 3-28 (1998).
Huang Jun-Xiong et al., “Applications of Preparative High-Performance Liquid Chromatography to the Separation and Purification of Peptides and Proteins,” Journal of Chromatography, 492, pp. 431-469 (1989).
Communication dated Nov. 23, 2006 enclosing the EP Search Report for EP Application No. 05255933.3 dated Nov. 9, 2006, and Annex to the EP Search Report, 4 pp.—Counterpart of U.S. Appl. No. 11/055,260.
Albouz-Abo, S., et al., A conformational study of the human and rat encephalitogenic myelin oligodendrocyte glycoprotein peptides 33-35, Eur. J. Biochem., 246, 59-70 (1997).
Becher, A., et al., Ectopically expressed—aminobutyric acid receptor B is functionally down-regulated in isolated lipid raft-enriched membranes, Biochemical and Biophysical Research Communications, 321, 981-987 (2004).
Becher, A., et al., The—aminobutyric acid receptor B, but not the metabotropic glutamate receptor type-1, associates with lipid rafts in the rat cerebellum, Journal of Neurochemistry, 79, 787-795 (2001).
Biel, M., et al., Tissue-specific expression of high-voltage-activated dihydropyridine-sensitive L-type calcium channels, Eur. J. Biochem, 200, 81-88 (1991).
Bledi, Y., et al., Proceed: A proteomic method for analysing plasma membrane proteins in living mammalian cells, Briefings in Functional Genomics & Proteomics, 2, 3, Research Library (Oct. 2003).
Blonder, J., et al., Enrichment of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry, Journal of Proteome Research, 1, 351-360 (2002).
Foster, L., et al., Unbiased quantitative proteomics of lipid rafts reveals high specificity for
Martosella James D.
Zolotarjova Nina I.
Agilent Technologies , Inc
Therkorn Ernest G
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