Methods and systems for high homologous recombination...

Details Methods and systems for high homologous recombination... Methods and systems for high homologous recombination...

2011-02-22

2011-02-22

Joike, Michele K (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023100

Reexamination Certificate

active

07892823

ABSTRACT:
Disclosed are vectors, kits and methods useful in the construction of recombinant cells and DNAs via enhanced efficiency homologous recombination. The vectors are targeting vectors that contain a gene-of-interest spliced between two ends that are homologous to a genome target site. The ends of the vector may be protected from exonuclease attack by deploying a cap, such as a hair pin structure. The vector is linked to a nuclear localization signal sequence, and preferably, a bait peptide that binds to RAD51, to facilitate homologous recombination. The vector may be deployed in myriad genetic transformation applications, such as site-directed mutagenesis, gene therapy, and the like.

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Cao et al. Killing effect of coexpressing cytosine deaminase and thymidine kinase on rat vascular smooth muscle cells. Chin Med J (Engl). Oct. 2004; 117(10):1464-70.
Galkin et al. BRCA2 BRC motifs bind RAD51-DNA filaments. Proc Natl Acad Sci U S A. Jun. 14, 2005;102(24):8537-42. Epub Jun. 3, 2005.
Shivji et al. A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchange. Nucleic Acids Res. 2006;34(14):4000-11. Epub Aug. 16, 2006.

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