Methods and systems for adding a reagent to an analyte in a gel

Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C204S456000, C204S465000, C204S466000, C204S615000, C436S086000, C436S087000, C436S094000, C436S174000, C435S018000, C435S023000, C435S024000

Reexamination Certificate

active

07989215

ABSTRACT:
The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

REFERENCES:
patent: 3378347 (1968-04-01), Saravis
patent: 3631575 (1972-01-01), Farris
patent: 3888759 (1975-06-01), Elson et al.
patent: 3932229 (1976-01-01), Grandine
patent: 4039247 (1977-08-01), Lawman et al.
patent: 4181594 (1980-01-01), Rizk et al.
patent: 4713349 (1987-12-01), Levin
patent: 4827780 (1989-05-01), Sarrine et al.
patent: 4834946 (1989-05-01), Levin
patent: 5100626 (1992-03-01), Levin
patent: 5217591 (1993-06-01), Gombocz et al.
patent: 5217592 (1993-06-01), Jones
patent: 5245185 (1993-09-01), Busch et al.
patent: 5340449 (1994-08-01), Shukla
patent: 5585275 (1996-12-01), Hudson et al.
patent: 5800785 (1998-09-01), Bochner
patent: 5840169 (1998-11-01), Andersen
patent: 6165541 (2000-12-01), Merchant et al.
patent: 6231813 (2001-05-01), Ally et al.
patent: 6342143 (2002-01-01), Minden
patent: 7364897 (2008-04-01), Heaney et al.
patent: 7645369 (2010-01-01), Hoeltke et al.
patent: 7736594 (2010-06-01), Grudzien et al.
patent: 2002/0096431 (2002-07-01), Sevigny et al.
patent: 2002/0146832 (2002-10-01), Michel et al.
patent: 2004/0178073 (2004-09-01), Kozulic
patent: 2003200406 (2003-08-01), None
patent: 0 382 426 (1990-08-01), None
patent: 1 048 949 (2000-11-01), None
patent: 99/22228 (1999-05-01), None
patent: 00/77510 (2000-12-01), None
patent: 02/071072 (2002-09-01), None
patent: 2004/044574 (2004-05-01), None
patent: W02004/063739 (2004-07-01), None
patent: WO 2005/029061 (2005-03-01), None
Aebersold, R., et al., “Mass spectrometry-based proteomics”. Nature (2003) 422, 198-207.
Breci, L., et al., “Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing”. Proteomics (2005) 5, 2018-2028.
Buzas, Z., et al., “Direct vertical electroelution of protein from a PhastSystem band for mass spectrometric identification at the level of a few picomoles”. Proteomics (2001) 1, 691-698.
Cargile, B., et al., “Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pl predictability of peptides”. Electrophoresis (2004) 25, 936-945.
Gevaert, K., et al., “Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides”. Nature Biotechnology (2003) 21, 566-569.
Gombocz, E., et al., “Electroelution of nonfluorescent stacked proteins detected by fluorescence optics from gel electrophoretic bands for transfer into mass spectrometry”. Electrophoresis (2000) 21, 846-849.
Heller, M., et al., “Two-stage Off-Gel isoelectric focusing: Protein followed by peptide fractionation and application to proteome analysis of human plasma”. Electrophoresis (2005) 26, 1174-1188.
Michel, P., et al., “Protein fractionation in a multicompartment device using Off-Gel(TM) isoelectric focusing”. Electrophoresis (2003) 24, 3-11.
Peng, J., et al., “Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC-MS/MS) for Large-Scale Protein Analysis: The Yeast Proteome”. Journal of Proteome Research (2003) 2, 43-50.
Sinha, P., et al., “Isoelectric focusing of basic proteases in immbolized pH gradients”. Journal of Biochemical and Biophysical Methods (1987) 15, 199-206.
Washburn, M., et al., “Large-scale analysis of the yeast proteome by multidimensional protein identification technology”. Nature Biotechnology (2001) 19, 242-247.
Yefimov, S., et al., “Sequential electroelution and mass spectroscopic identification of intact sodium dodecyl sulfate-proteins labeled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester”. Electrophoresis (2001) 22, 2881-2887.
Yi, E., et al., “Increased quantitative proteome coverage with 13C/12C-based, acid-cleavable isotope-coded affinity tag reagent and modified data acquisition scheme”. Proteomics (2005) 5, 380-387.
Zuo, X., et al., “Microscale Solution Isoelectrofocusing”. Methods in Molecular Biology, vol. 244: Protein Purification Protocols: Second Edition (2004) 361-375.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods and systems for adding a reagent to an analyte in a gel does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and systems for adding a reagent to an analyte in a gel, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and systems for adding a reagent to an analyte in a gel will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2636045

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.