Methods and solvent vehicles for reagent delivery in oligonucleo

Details Methods and solvent vehicles for reagent delivery in oligonucleo Methods and solvent vehicles for reagent delivery in oligonucleo

1996-12-13

1999-02-23

Marschel, Ardin H.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, 525 50, 525 53, 525 542, C07H 1900, C08G 6348

Patent

active

058745548

ABSTRACT:
Solvent vehicles and methods of their use in the pulse jet delivery of reagents in automated oligonucleotide synthesis by the solid-phase phosphoramidite method are provided. For pulse jet delivery of the activated monomer, acetonitrile in combination with at least one stabilizer co-solvent is employed as the solvent vehicle, where the stabilizer co-solvent is an inert, organic solvent having a boiling point of at least 100.degree. C. For pulse jet delivery of the detritylation reagent, a mono- or disubstituted methane is employed as the solvent vehicle, where substituents have a molecular weight greater than 36 daltons. Use of the solvent vehicles according to the subject invention provides for reduced reagent precipitation in the pulse jet tubes and improved results.

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