Methods and reagents for typing HLA class I genes

Details Methods and reagents for typing HLA class I genes Methods and reagents for typing HLA class I genes

1995-10-03

2000-08-15

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, C12Q 168, C07H 2102

Patent

active

061034657

ABSTRACT:
A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4855225 (1989-08-01), Fung et al.
patent: 5424184 (1995-06-01), Santamaria et al.
patent: 5451512 (1995-09-01), Apple et al.
Fernandex-Vina et al., Human Immunology 33: 163-173 (1992), DNA typing for HLA class I Alleles: I. Subsets of HLA-A2 and of -A28.
Allen et al., Human Immunology 40: 25-32 (1994), A comprehensive polymerase chain reaction-oligonucleotide typing system for the HLA class I A locus.
Guttridge et al., Tissue Antigens 44: 43-46 (1994), Identification of HLA-B35, B53, B18, B5, B78, and B17 alleles by the polymerase chain reaction using sequence-specific primers (PCR-SSP).
Yoshida et al., Human Immunology 34: 257-266 (1992), Polymerase-chain-reaction-based analysis of polymorphism in the HLA-B gene.
Dominguez et al., Immunogenetics 36: 277-282 (1992), Molecular typing of HLA-B27 alleles.
Andrien et al., Tissue Antigens 42: 480-487 (1993), HLA-B locus DNA typing: detection of B*7801 and seven additional alleles by BW6-specific exon 2 amplification.
Bunce et al., Tissue Antigens 43: 7-17 (1994), Rapid DNA typing for HLA-C using sequence-specific primers(PCR.sub.-- SSP): identification of serologically defined HLA-C alleles including several new alleles.
Janeway Jr., Scientific American, Sep., 73-79 (1993), How the immune system recognizes invaders.
Petersdorf et al., Tissue Antigens 44: 93-99 (1994), Molecular diversity of the HLA-C locus in unrelated marrow transplantation.
Santamaria et al., Human Immunology 33: 69-81 (1992), HLA class II "typing": direct sequencing of DRB, DQB, and DQA genes.
Santamaria et al., Human Immunology 37: 39-50 (1993), HLA class I sequence-based typing.
Petersdorf et al., Tissue Antigens 44: 211-216 (1994), Analysis of HLA-B*44 alleles encoded on extended HLA haplotypes by direct automated sequencing.
Cereb et al., Tissue Antigens 45: 1-11 (1995), Locus-specific amplification of HLA class I genes from genomic DNA: locus-specific sequences in the first and third introns of HLA-A, -B, and -C alleles.
Gelfand and White, PCR Protocols: A Guide to Methods and Applications, ed., Innis et al., Academic Press, CA, pp. 129-141 (1991), Thermostable DNA polymerases.
Chou et al, Nucleic Acids Research 20(7):1717-23 (1992) Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.
Engelke et al., Proceedings of the National Academy of Sciences 85: 544-548 (1988), Direct sequencing of enzymatically amplified human genomic DNA.
McBride et al., Clinical Chemistry 35(11): 2196-2201 (1989), Automated DNA sequencing methods involving polymerase chain reaction.
Gorman et al., BioTechniques 7(4): 326-329 (1989), Simplified method for selective amplification and direct sequencing of cDNAs.
Trowsdale et al., Immunology Today 12(12): 443-446 (1991), Map of the human MHC.
Miller et al., Nucleic Acids Research 16(3): 9-10 (1988), A simple salting out procedure for extracting DNA from human nucleated cells.
Geraghty, Proceedings of the Eleventh International Histocompatibility Workshop and Conference vol. 2, Tsuji et al. eds., pp. 129-132, Oxford University Press (1992), The HLA class I gene family.
Applied Biosystems, 373A DNA Sequencing System User's Manual, Part No. 901156, Software Version 1.10, Document Rev. C, Jan. 1992.
Holtz et al., DNA-Technology and Its Forensic Application, Berghaus et al. eds., pp. 79-84, Springer-Verlag, Berlin (1991), DNA Typing Using PCR Amplified Fragments in the HLA Class I Region.
Lee et al., Nucleic Acids Research 20(10): 2471-2483 (1992), DNA sequencing with dye-labeled terminators and T7 polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Prober et al., Science 238: 336-341 (1987), A system for rapid DNA sequencing with fluorescent chain-terminating, dideoxynucleotides.
Lawyer et al., J. Biol. Chem. 264:6427-6437 (1989), Isolation, characterization, and expression in Escherichia Coli of the DNA polymerase gene from Thermus aquaticus.
Krausa, P. et al., Tissue Antigens, 45(4): 223-31 (Apr. 1995), Genetic Polymorphism within HLA-A *02: significant allelic variation revealed in different populations.
Blasczyk, R. et al., Tissue Antigens, 46(2): 86-95 (Aug. 1995), Complete subtyping of the HLA-A locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis.
Kennedy, L. J. et al., Tissue Antigens, 46(2): 187-195 (1995), Definition of HLA-C alleles using sequence-specific oligonucleotide probes (PCR-SSOP) .

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