Methods and reagents for typing HLA Class I genes

Details Methods and reagents for typing HLA Class I genes Methods and reagents for typing HLA Class I genes

1995-12-22

2000-02-29

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168

Patent

active

060307757

ABSTRACT:
Consensus sequences of introns 1, 2 and 3 from the majority of HLA-A, -B and -C allotypes are identified and used to develop primers located within introns 1 and 3 of the HLA-A, HLA-B and HLA-C genes. These primers are suitable for locus-specific amplification of the entirety of exons 2 and 3, i.e., the portion of these of genes most suitable for use in typing of HLA-A, HLA-B and HLA-C. These primers are also suitable for use as sequencing primers to determine the HLA alleles in sequence-based HLA typing. Thus, the primers can be used for testing a sample to determine the HLA-A, -B or -C type of the sample by treating the tissue sample to obtain nucleic acid polymers suitable for amplification; combining the nucleic acid polymers with a first primer which is complementary to a portion of intron 1 or intron 3 of the HLA gene, and a second primer which is complementary to some other portion of the HLA gene under conditions suitable for amplification to obtain an amplified product; and evaluating the amplified product to determine the allelic type of the HLA-A, HLA-B or HLA-C genes. Preferably, at least one of the amplification primers has a sequence which provides locus-specific amplification.

REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 5424184 (1995-06-01), Santamaria et al.
1988 Stratagene Catalog (published by Stratagene, 11011 North Torrey Pines Rd., La Jolla, California 92037), 1988.
Gao et al. "Characterization of HLA-A Polymorphism by Locus-Specific Polymerase Chain Reaction Amplification and Oligonucleotide Hybridization" Human Immunology 41: 267-279 (1994).
Joseph et al., "Classification of Mutations at the HLA-A Locus by Use of the Polymerase Chain Reaction", Environmental and Molecular Mutagenesis 22: 152-156 (1993).
Lawlor et al., "Ancient HLA genes from 7,500 year-old archaelogical remains", nature 349: 785788 (1991).
Malissen et al., "Exon/Intron Organization and Complete Nucleotide Sequence of an HLA Gene", Proc. Nat'l Acad. Sci. USA 79: 893-897 (1982).
Geraghty et al., "The HLA Class I Gene Family Includes at Least Six Genes and Twelve Pseudogene and Gene Fragments" J. Immunology 149: 1934-1946 (1992).
Blasczyk et al., "Complete subtyping of the HLA-A Locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis", Tissue Antigens 46: 86-95 (1995).
Cereb, N., et al., Tissue Antigens 1995, 45: 1-11.
Summers, C.W., et al., European Journal of Immunogenetics 1993, 20: 201-240 .

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