Methods and reagents for the polymerase chain reaction amplifica

Details Methods and reagents for the polymerase chain reaction amplifica Methods and reagents for the polymerase chain reaction amplifica

1994-02-25

1996-04-30

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435183, 435194, C12P 1934, C12N 900, C12N 912

Patent

active

055124623

ABSTRACT:
Methods and reagents are provided for the amplification of DNA sequences longer than 10 kilobases by the polymerase chain reaction (PCR). The methods use compositions consisting of a primary thermostable DNA polymerase from Thermus thermophilus combined with a lesser amount of a secondary thermostable DNA polymerase possessing a 3'-to-5' exonuclease activity from Thermococcus litoralis, Pyrococcus species GB-D or Thermotoga maritima. The DNA polymerase compositions, when used with the disclosed reaction buffer, enable amplifications of DNA sequences up to at least 42.2 kilobases in length.

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