Methods and reagents for staining intracellular components

Bleaching and dyeing; fluid treatment and chemical modification – Dye or potential dye composition – additive – treatment,... – Polymeric dye – e.g. – a chromophore pendant from an addition...

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8554, 8648, 8517, C09D 6910

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050530542

ABSTRACT:
Reagents and methods for intracellular staining of anionic materials as provided. The inherent staining characteristics of a fluorescent molecule may be suitably altered by covalently linking a plurality of said molecules to a nonimmunoglobulin derived cationic polymer having the desired staining specificity. By careful selection of the net charge and size of the dye polymer complex, diffusion through the cellular membrane can be enhanced and an enhanced fluorescent signal obtained by localization of a plurality of fluorescent molecules at the site of interest.

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