Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-11-15
1999-11-30
Johnson, Nancy A
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 72, 435 723, 435332, 5303871, 5303877, 5303882, 5303888, 53038885, 5303891, 5303897, 5303913, G01N 3353, C12N 520, C07K 1628
Patent
active
059940883
ABSTRACT:
This invention relates to immunological reagents and methods specific for a mammalian, transmembrane protein termed Pgp, having a non-specific efflux pump activity established in the art as being a component of clinically-important multidrug resistance in cancer patients undergoing chemotherapy. The invention provides methods for developing and using immunological reagents specific for certain mutant forms of Pgp and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP depleting agents. The invention also provides improved methods for purifying hematopoietic stems cells expressing Pgp and diagnostic and therapeutic methods for cancer cells expressing Pgp.
REFERENCES:
patent: 4837306 (1989-06-01), Ling et al.
patent: 4925787 (1990-05-01), Tanihara et al.
patent: 5057598 (1991-10-01), Pollack et al.
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5130127 (1992-07-01), Herlyn
patent: 5132405 (1992-07-01), Huston et al.
patent: 5134075 (1992-07-01), Hellstrom et al.
patent: 5204095 (1993-04-01), Goodall et al.
patent: 5206352 (1993-04-01), Roninson et al.
patent: 5215913 (1993-06-01), Posner
patent: 5434075 (1995-07-01), Mechetner et al.
patent: B14918163 (1993-04-01), Young et al.
Akiyama et al., "Most Drugs that Reverse Multidrug Resistance Also Inhibit Photoaffinity Labeling of P-Glycoprotein by a Vinvlastine Analog," Molec. Pharm. 33:144 (1988).
Arcesi et al., "Monoclonal Antibody to an External Epitope of the Human mdrl P-Glycoprotein," Cancer Res. 53:310-317 (1993).
Ball et al., 1990, "Correlation of CD34 and Multidrug Resistance P170 with Fab and Cytogenetics but not Prognosis in Acute Myeloid Leukemia (AML)," Blood 76(10, Suppl 1):252a.
Barth et al., "Interferon .gamma. and Tumor Necrosis Factor Have a Role in Tumor Regressions Mediated by Murine CD8 Tumor-infiltrating Lymphocytes," 1991, J. Exp. Med. 173:647-658.
Beck, "Do Anti-P-Glycoprotein Antibodies Have a Future in the Circumvention of Multidrug Resistance?" J. Natl. Cancer Inst. 83:364 (1991).
Berke, 1989, "Functions and Mechanisms of Lysis Induced by Cytotoxic T Lymphocytes and Natural Killer Cells," in Paul, ed., Fundamental Immunology, 2nd ed., Raven Press, N.Y., pp. 753-764.
Bertoncello et al., 1985, "Multiparameter Analysis of Transplantable Hemopoietic Stem Cells Homing to Marrow and Spleen on the Basis of Rhodamine-123 Fluorescence," Exp. Hematol. 13:999-1006.
Broxterman et al., "Increase of Daunorubicin and Vincristine Accumulation in Multidrug Resistant Human Ovarian Carcinoma Cells by a Monoclonal Antibody Reacting with P-Glycoprotein," Biochem. Pharm. 37:2389-92 (1988).
Burgess and Kelly, "Constitutive and Regulated Secretion of Proteins," Annu. Rev. Cell Biol. 3:243-294 (1987).
Burgess et al., "Possible Dissociation of the Heparin-binding and Mitogenic Activities of Heparin-binding (Acidic Fibroblast) Growth Factor-1 from Its Receptor-binding Activities by Site-directed Mutagenesis of a Single Lysine Residue," Journal of Cell Biology 111:2129 (1990).
Carter et al., "Improved oligonucleotide site-directed mutagenesis using M13 vectors," Nucleic Acids Res. 13:4431-4443 (1985).
Cenciarelli et al., "Characterization by Somatic Cell Genetics of a Monoclonal Antibody to the MDR1 Gene Product (P-Glycoprotein): Determination of P-Glycoprotein Expression in Multi-Drug Resistant KB and CEM Cell Variants," Int. J. Cancer 47:533 (1991).
Chaudhary et al., "Expression and Activity of P-Glycoprotein, a Multidrug Efflux Pump, in Human Hematopoietic Stem Cells," Cell 66:85 (1991).
Chaudhary et al., "Expression and Activity of the Multidrug Resistance P-Glycoprotein in Human Peripheral Blood Lymphocytes," Blood 80:2735 (1992).
Chen et al., "Internal Duplication and Homology with Bacterial Transport Proteins in the mdrl (P-Glycoprotein) Gene from Multidrug-Resistant Human Cells," Cell 47:381 (1986).
Choi et al., "An Altered Pattern of Cross-Resistance in Multidrug-Resistant Human Cells Results from Spontaneous Mutations in the mdrl (P-Glycoprotein) Gene," Cell 53:519-529 (1988).
Choi et al., "Multidrug resistance after retroviral transfer of the human MDR1 gene correlates with P-Glycoprotein density in the plasma membrane and is not affected by cytotoxic selection," Proc. Natl. Acad. Sci. USA 88:7386-7390 (1991).
Co & Queen, 1991, "Humanized antibodies for Therapy," Nature 351:501-502.
Coon et al., "Multidrug Resistance Activity in Human Lymphocytes," Human Immunol. 32:134 (1991).
Cordon-Cardo et al., "Expression of the Multidrug Resistance Gene Product (P-Glycoprotein) in Human Normal and Tumor Tissues," J. Histochem. Cytochem. 38:1277 (1990).
Cornwell et al., "Increased Vinblastine Binding to Membrance Vesicles from Multidrug-resistant KB Cells," J. Bio. Chem. 261:7921 (1986).
Dillman et al., "Monoclonal Antibodies for Treating Cancer," Annals of Internal Med. 111(7):592 (1989).
Drach et al., "Subpopulations of Normal Peripheral Blood and Bone Marrow Cells Express a Functional Multidrug Resistant Phenotype," Blood 80:2729 (1992).
Efferth et al., 1989, "Reciprocal Correlation Between Expressionof P-Glycoprotein and Accumulation of Rhodamine 123 in Human Tumors," Anti Cancer Research 9:1633-1638.
Endicott & Ling, "The Biochemistry of P-Glycoprotein-Mediated Multidrug Resistance," Annu. Rev. Biochem. 58:137 (1989).
Ford et al., "Pharmacology of Drugs That Alter Multidrug Resistance in Cancer," Pharm. Rev. 42:155 (1990).
Georges et al., "Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodies," PNAS (USA) 87:152-56 (1990).
Gillies et al., "Antigen binding and biological activities of engineered mutant chimeric antibodies with human tumor specificities," Human Antibod. Hybridons 1(1):47 (1990).
Gillis, 1989, "T-Cell-Derived Lymphokines," in Paul, ed., Fundamental Immunology, 2d ed., Raven Press, N.Y., pp. 621-638.
Gottesman & Pastan, "Biochemistry of Multidrug Resistance Mediated By The Multidrug Transporter," Annu. Rev. Biochem. 62:385 (1993).
Gros et al., "Isolation and characterization of DNA sequences amplified in multidrug-resistant hamster cells," Proc. Natl. Acad. Sci. USA 83:337 (1986).
Gupta et al., "P-Glycoprotein (MDR 1 Gene Product) in Cells of the Immune System: Its Possible Physiologic Role and Alteration in Aging and Human Immunodeficiency Virsu-1 (HIV-1) Infection," J. Clin. Immunol. 13:289 (1993).
Hamada et al., "Functional Role for the 170-to 180kDa glycoprotein specific to drug-resistant tumor cells as revealed by monoclonal antibodies," PNAS 83:7785-7789 (1986).
Hamada et al., 1990, "Mouse-Human Chimeric Antibody against the Multidrug Transporter P-Glycoprotein," Cancer Res. 50:3167.
Harlow et al., Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press: New York, 1989.
Heike et al., "Monoclonal Anti-P-glycoprotein Antibody-dependent Killing of Multidrug-resistant Tumor Cells by Human Mononuclear Cells," Jpn. J. Cancer Res. 81:1155-61 (1990).
Higgins, "ABC Transporters: From Microorganisms to Man," Annu. Rev. Cell Biol. 8:67 (1992).
Hoogenboom et al., "Multi-subunit proteins on the surface of filamentous phage: methodoliges for displaying antibody (Fab) heavy and light chains," Nuceleic Acids Research 19(15):4133-4137.
Horio et al., "ATP-dependent transport of vinblastine in vesicles from human multidrug-resistant cells," Proc. Natl. Acad. Sci. USA 84:3580 (1988).
Huse, "Combinatorial Antibody Expression Libraries in Filamentous Phages," in Antibody Engineering, Carl Borreback ed. 1992, p. 103.
Kearny et al., "A New Mouse Myeloma Cell Line That Has Lost Immunoglobulin Expression But Permits The Construction of Antibody-Secreting Hybrid Cell Lines," J. Immunol. 123:1548 (1979).
Klimecki et al., "P-Glycoprotein Expression and Functionin Circulating Blood Cells From Normal Volunteers," Blood 83:2451 (1994).
Kobayashi et al., "Expression and Function of Multidrug Resistance P-Glycoprotein in a Cultured Natural Killer Cell-Rich Population Revealed by MRK16 Monoclonal Antibody and AHC-52," Biochem. Pharmacol. 48:1641 (1994).
Kronke et al., "Cyclosporin A inhibits T-cell growth factor ge
Mechetner Eugene
Roninson Igor B.
Board of Trustees of the University of Illinois
Johnson Nancy A
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