Methods and reagents for HLA class I A locus DNA typing

Details Methods and reagents for HLA class I A locus DNA typing Methods and reagents for HLA class I A locus DNA typing

1993-09-28

1995-09-19

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 235, 536 2433, C12P 1934, C12Q 168, C07H 2104

Patent

active

054515122

ABSTRACT:
Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I A gene and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples from a variety of sources and for detecting allelic variants not distinguishable by serological methods. This HLA-A DNA typing system can be used in a forward or reverse dot-blot format that is simple and rapid to perform, produces detectable signals in minutes, and can be used for tissue typing, determining individual identity, and identifying disease susceptible individuals.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
Parham et al, J. Immunology 142:3937-395 (1989).
Malissen et al., Feb., 1982, "Exon/Intron Organization and Complete Nucleotide Sequence of an HLA Gene" Proc. Natl. Acad. Sci. USA 79:893-897.
Saiki et al., Nov., 1986, "Analysis of Enzymatically Amplified B-Globin and HLA-DQalpha DNA With Allele-Specific Oligonucleotide Probes" Nature 324(13):163-166.
Bjorkman and Parham, 1990, "Structure, Function, and Diversity of Class I Major Histocompatibility Complex Molecules" Annu. Rev. Biochem. 59:253-288.
Zemmour and Parham, 1991, "HLA Class I Nucleotide Sequences, 1991" Immunogenetics 33:310-320.
Baxter-Lowe et al., Aug., 1989, "HLA Gene Amplification and Hybridization Analysis of Polymorphism HLA Matching for Bone Marrow Transplantation of a Patient With HLA-Deficient Severe Combined Immunodeficiency Syndrome" J. Clin. Invest. 84:613-618.
Lawlor et al., Feb. 1991, "Ancient HLA Genes From 7,500-Year-Old Archaeological Remains" Nature 349:785-788.
Ennis et al., Apr., 1990, "Rapid Cloning of HLA-A, B cDNA Using the Polymerase Chain Reaction: Frequency and Nature of Errors Produced in Amplification" Proc. Natl. Acad. Sci. USA 87:2833-2837.
Hill et al., 1991, "HLA Class I Typing by PCR: HLA-B27 and an African B27 Subtype" Lancet 337:640-642.
Fernandez-Vina et al., 1992, "DNA Typing for HLA Class I Alleles: I. Subsets of HLA-A2 and of -A48" Human Immunology 33:163-173.
Browning et al., 1993, "Tissue Typing the HLA-A Locus From Genomic DNA by Sequenc-Specific PCR: Comparison of HLA Genotype and Surface Expression on Colorectal Tumor Cell Lines" Proc. Natl. Acad. Sci. USA 90:2842-2845.
Oh et al., 1993, "Isoelectric Focusing Subtypes of HLA-A can be Defined by Oligonucleotide Typing" Tissue Antigens 41:135-142.
Yoshida et al., 1992, "Polymerase-Chain Reaction-Based Analysis of Polymorphism in the HLA-B Gene" Human Immunology 34:257-266.

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