Methods and reagents for .gamma.-globin typing

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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935 77, 935 78, C12Q 168

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active

052738833

ABSTRACT:
Methods and reagents for determining an individual's genotype at the .sup.G .gamma.-globin locus with respect to a set of three alleles using sequence-specific oligonucleotide probes enable one to type samples from a variety of sources, including samples comprising RNA or cDNA templates, and can be applied to nucleic acids in which a target region spanning the polymorphism has first been amplified. This three-allele system facilitates typing tissue for determining individual identity and has application in the field of forensic science.

REFERENCES:
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
Jeffreys, 1979, Cell 18:1-10, "DNA Sequence Variants in the G-Gamma-, A-Gamma-, Delta-and Beta-Globin Genes of Man".
Slightom et al., 1980, Cell 21:627-638, "Human Feta G-Gamma-and A-Gamma-Globin Gene: Complete Nucleotide Sequences Suggest that DNA can be Exchanged Between These Duplicate Genes".
Shiokawa et al., 1989, J. Biochem. 105:184-189, "Heterogeneity of the Gamma-Globin Gene Sequences in Japanese Individuals: Implication of Gene Conversion in Generation of Polymorphisms".
Cui et al., 1989, Proc. Natl. Acad. Sci. USA 86:9389-9393, "Single-Sperm Typing: Determination of Genetic Distance Between the G-Gamma-Globin and Parathyroid Hormone loci by Using the Polymerase Chain Reaction and Allele-Specific Oligomers".
Higuchi et al., 1988, Nature 332:543-546, "DNA Typing From Single Hairs".
Li et al., 1988, Nature 335:414-417, "Amplification and Analysis of DNA Sequences in Single Human Sperm and Diploid Cells".
Reynolds and Sensabaugh, 1991, Anal. Chem. 63:2-15, "Analysis of Genetic Markers in Forensic DNA Samples Using the Polymerase Chain Reaction".
Saiki et al., 1989, Proc. Natl. Acad. Sci. USA 86:6230-6234, "Genetic Analysis of Amplified DNA With Immobilized Sequence-Specific Oligonucleotide Probes".
AmpliType DQalpha DNA Typing Kit Package Insert.

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