Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-12-21
1999-07-06
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 2432, 435 911, 435 912, C12Q 168, C07H 2102, C07H 2104, C12P 1934
Patent
active
059196173
ABSTRACT:
The present invention provides novel methods and materials for detecting the presence of a fungus in a biological sample. The inventive methods and materials exploit the fact that the amino acid sequence of the saccharopine dehydrogenase molecule is highly conserved in fungi. Inventive hybridization probes, nucleic acids, PCR primers, antibodies, epitopes, reagents and methods are provided.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5075216 (1991-12-01), Innis et al.
patent: 5079352 (1992-01-01), Gelfand et al.
Garrad et al.Infection and Immunity 62: 5027-5031 1994.
Xuan et al. Molecular and Cellular Biology 10: 4795-4806 1990.
Garrad and Bhattacharjee J. of Biology 174: 7379-7384 1992.
Ford et al. J. of Basic Microbiology 33: 179-186 1993.
Rothwell, in Understanding Genetics: A Molecular Approach 1993.
Musial, et al., Fungal Infections of the Immunocompromised Host: Clinical and Laboratory Apsects, Clin. Microbiol. Rev. 1:349-364 (1988).
Hopfer, R.L., et al., Detection and differentation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis, J. Med. Vet. Pharm. 31:65-75 (1993).
Buchman, T.G., et al., Detection of surgical pathogens by in vitro DNA amplification. Part I, Rapid identification of Candida albicans by in vitro amplification of a fungal specific gene. Surgery, 108:338-347 (1990).
Bhattacharjee, The .alpha.-aminoadipate Pathway for the Biosynsethesis of Lysine in Lower Eukaryotes, CRC Critical rev. in Microbiol. 12:131-151 (1985).
Lejohn, Enzyme Regulation, Lysine Pathways and Cell Wall Structures as Indicators of Major Lines of Evolution in Fungi, Nature 231:164-168 (1971).
Vogel, Two Modes of Lysine Synthesis Among Lower Fungi: Evolutionary Significance, Biochim. Biophys, Acta 41:172-174 (1960).
Garrad and Bhattacharjee, Lysine biosynthesis in selected pathogenic fungi: Characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans, J. Bacteriol. 174:7379-7384 (1992).
Garrard, R., et al. (1994), "Molecular and Functional Analysis of the LYS1 Gene of Candida albicans," Infection and Immunity, vol. 62., No. 11, pp. 5027-5031.
Goshorn, A.K., et al. (1992), "Gene Isolation by Complementation in Candida albicans and Applications to Physical and Genetic Mapping," Infection and Immunity, vol. 60, No. 3, pp. 876-884.
Xuan, J.W. et al., (1990), "Overlapping Reading Frames at the LYS5 Locus in the Yeast Yarrow lipolytica," Molecular and Cellular Biology, vol. 10, No. 9, pp. 4795-4806.
Database Medline, A.N. 930554354, XP002021825 (1992).
Bhattacharjee Jnanendra K.
Garrad Richard C.
Peery Robert B.
Skatrud Paul L.
Fredman Jeffrey
Miami University
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