Methods and reagents for detecting endotoxin

Details Methods and reagents for detecting endotoxin Methods and reagents for detecting endotoxin

2005-02-01

2005-02-01

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

Reexamination Certificate

active

06849426

ABSTRACT:
A reagent containing a purified horseshoe crab Factor C, particularly a recombinantly produced Factor C, and a surfactant can be used in a sensitive, rapid, and reproducible assay to detect endotoxin.

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Nakamura et al., “Intracellular serine-protease zymogen, factor C, from horseshoe crab hemocytes. Its activation by synthetic lipid A analogues and acidic phospholipids”, Eur. J. Biochem, 1988, pp 89-94, vol. 176(1).
Tan et al., “High-affinity LPS binding domain(s) in recombinant factor C of a horseshoe crab neutralizes LPS-Induced lethality”, The FASEB Journal, May 2000, 859-870, vol. 14.
Tokunaga et al, “Further Studies on Lipopolysaccharide-Sensitive Serine Protease Zymogen (Factor C): Its Isolation from Limulus polyphemus Hemocytes and Identification as an Intracellular Zymogen Activated by α-Chymotrypsin, Not by Trypsin”, J. Biochem., 1991, pp 150-157, vol. 109.
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