Methods and reagents for cyanide-free determination of hemoglobi

Details Methods and reagents for cyanide-free determination of hemoglobi Methods and reagents for cyanide-free determination of hemoglobi

1997-05-27

1999-09-28

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 8, 436 10, 436 15, 436 17, 436 18, 436 66, 436164, 436166, 436174, 436175, 435 2, 424 9371, 424534, G01N 3348

Patent

active

059587814

ABSTRACT:
Cyanide-free reagents for the determination of hemoglobin and leukocytes present in a blood sample contain an aqueous solution of at least one quaternary ammonium salt, preferably selected from the group of alkyltrimethylammonium salts, alkyldimethylbenzylammonium salts or alkylpyridium salts consisting of tetradecyltrimethyl ammonium bromide (TTAB), dodecyltrimethyl ammonium chloride, cetyltrimethyl ammonium bromide, hexadecyltrimethyl ammonium bromide and benzalkonium chlorides, and hydroxylamine salts, especially hydrochloride, sulfate and phosphates and other acid salts. The method involves mixing the reagent with a diluent- diluted blood sample, presenting it to an absorbance spectrophotometer and measuring the resulting optical density as an indicator of hemoglobin concentration. This cyanide-free reagent could be used solely for hemoglobin determinations, or , it can also be used in leukocyte counting and sizing using hematology instrumentation.

REFERENCES:
patent: 3652222 (1972-03-01), Denney et al.
patent: 3874852 (1975-04-01), Hamill
patent: 4185964 (1980-01-01), Lancaster
patent: 4200435 (1980-04-01), Stroupe et al.
patent: 4255385 (1981-03-01), Stroupe et al.
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4521518 (1985-06-01), Carter et al.
patent: 4853338 (1989-08-01), Benezra et al.
patent: 4962038 (1990-10-01), Carter et al.
patent: 5227304 (1993-07-01), Wong
patent: 5242832 (1993-09-01), Sakata et al.
patent: 5259437 (1993-11-01), Toda et al.
patent: 5336518 (1994-08-01), Narayanan et al.
patent: 5612223 (1997-03-01), Kim et al.
patent: 5763280 (1998-06-01), Li et al.
patent: 5786224 (1998-07-01), Li et al.
Oshiro, et al. "New Method for Hemoglobin Determination by Using Sodium Laury Sulfate (SLS)", Clin. Biochem. 15 (1); 83-88; 1982.
Oshiro, et al. "Sodium Lauryl Sulfate-Hemoglobin"; Sysmex Journal; vol. 13, No. 2; 220-227; 1990.
Bunn & Forget; "Hemoglobin: Molecular, Genetic and Clinical Aspects"; W. B. Saunders Co.; 638-644; 1986.
Merck Index; 10th Edition; 703, 1100, Date Unknown.
Database WPI, Week 9318, Derwent Publication Ltd., London, GB; AN 93-149936 & SU,A,1 707 544 (Onocology Res. Inst.) Jan. 23, 1992.
Patent Abstracts of Japan, vol. 11, No. 107 (P-563), Apr. 4, 1987, JP61254862, Nov. 12, 1986.
Aldrich Catalog; 1992-1993 Edition; 701.

Affiliated with

Also associated with

Rating

Methods and reagents for cyanide-free determination of hemoglobi does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and reagents for cyanide-free determination of hemoglobi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and reagents for cyanide-free determination of hemoglobi will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-702534