Compositions – Ammonia – alkali or base – bindant containing
Patent
1994-06-08
1996-05-21
Lilling, Herbert J.
Compositions
Ammonia, alkali or base, bindant containing
435 911, 536 253, 536 2531, 536 268, 536 2851, C12P 1934, C07H 1910, C07H 1906, C07H 19073
Patent
active
055186513
ABSTRACT:
Disclosed are reagents of methods for cleaving and deprotecting insolubilized and protected synthetic oligonucleotides. In a particularly preferred embodiment, the reagent comprises methylamine and t-butylamine.
REFERENCES:
patent: 4033977 (1977-07-01), Philipp et al.
patent: 4242097 (1980-12-01), Rich et al.
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4450277 (1984-05-01), Graf et al.
patent: 4680295 (1987-07-01), Fowler et al.
patent: 5001264 (1991-03-01), Saeki et al.
patent: 5296023 (1994-03-01), Gregory et al.
Adrich Catalogue 1988-1989 p. 983 "Methylamine 40 wt % Solution in Water" #M2,775-1.
Bhat, V. et al "A Simple and Convenient Method for the Selective N-Acylatrims of Cytosine Nucleotides" Nucleosides & Nucleotides 8(2): 179-183 (1989).
Chaix, C., et al. "The Use of Labile Base Protecting Groups in Oligonucleotide Synthesis" Tetrahedron Letters 30(1): 71-74 (1989).
Singh, R. K., et al. "Protecting Groups Used in Oligonucleotide Synthesis: A Current Survey" J. Sci. & Ind. Res. 49: 441-448 (1990).
Koster, H. "N-Acyl Protecting Groups for Deoxynucleotides: A Quantitative and Comparative Study" Tetrahedron 37: 363-369 (1981).
Chaix, C., et al. "Solid Phase Synthesis of the 5'-Half of the Initiator t-RNA From B. Subtilis" Nuc. Acid Res. 17(18): 7381-7393 (1989).
Weber H. & Khorana, H. G. "CIV. Total Synthesis of the Structured Gene for an Alanine Transfer Ribonucleic Acid From Yeast" J. Vol. Biol. 72: 219-249 (1972).
Vu, H., et al "Fast Oligonucleotide Deprotection Phosphoramidite Chemistry for DNA Synthesis" Tetrahedron Letters 31(50): 7269-7272 (1990).
Sinha, N. D., et al. "Polymer Support Oligonucleotide Synthesis XVIII" Nuc. Acids Res. 12(11): 4539-4556 (1984).
McBride, L. J. & Caruthers, M. H. "An Investigation of Several Oligonucleotide Phosphoramidites Useful for Synthesizing Deoxynucleotides" Tetrahedron Letters 24(3): 245-248 (1983).
Hanna Naeem B.
Reddy Parameswara M.
Beckman Instruments Inc.
Grant Arnold
Henry Janis C.
Lilling Herbert J.
May William H.
LandOfFree
Methods and reagents for cleaving and deprotecting oligonucleoti does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and reagents for cleaving and deprotecting oligonucleoti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and reagents for cleaving and deprotecting oligonucleoti will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2035456