Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1996-11-12
1998-11-03
Wallenhorst, Maureen M.
Chemistry: analytical and immunological testing
Biological cellular material tested
436 10, 436 17, 436 18, 436116, 436174, 435 2, 435 29, 435 30, G01N 3348
Patent
active
058307648
ABSTRACT:
The invention describes improved methods and reagent compositions employed therein to determine the existence of blood disorders such as hemolytic anemias in a blood sample obtained from an individual. The methods are preferably carried out on an automated flow cytometer and encompass determining if the individual's red blood cells have undergone a loss of membrane surface area by providing measurements of the surface area and the sphericity of the red blood cells in a whole blood sample as a function of osmolality. The method and reagent compositions used therein provide for the first time accurate measurements of the surface areas of both the mature red blood cell and the reticulocyte populations in a whole blood sample. The method and reagents of the invention promise to shed new insights into reticulocyte membrane remodeling in various red cell disorders.
REFERENCES:
patent: 4412004 (1983-10-01), Ornstein et al.
patent: 4575490 (1986-03-01), Ornstein et al.
patent: 4735504 (1988-04-01), Tycko
patent: 5284771 (1994-02-01), Fan et al.
patent: 5350693 (1994-09-01), Colella et al.
patent: 5411891 (1995-05-01), Fan et al.
patent: 5633167 (1997-05-01), Fan et al.
Lew et al. "Generation of normal human red cell volume . . . " Blood, vol. 86, No. 1, pp. 334-341, 1995.
Ponder, E. Hemolysis and related phenomenon. Grune & Stratton, New York. 1948 reissued 1971. pp. 10-26.
Evans EA and Fung YC. Improved measurements of the erythrocyte geometry. Microvasc. Res. 4: 335, 1972.
Engstrom KG, Moller B. and Meiselman HJ. Optical evaluation of red blood cell geometry using micropipette aspiration. Blood Cells 18: 241-258, 1992.
Engstrom KG and Meiselman HJ. Optical and mathematical corrections of micropipette measurements of red blood cell geometry during anisotonic perfusion. Cytometry 17: 279-286, 1994.
Nash GB and Wyard SJ. Changes in surface area and volume measured by micropipette aspiration for erthrocytes aging in vivo. Biorheology 17: 479-848, 1980.
Clark MR, Mohandas N, and Shohet SB. Osmotic gradient ektacytometry: comprehensive characterization of red cell volume and surface maintenance. Blood 61: 899-910, 1986.
Bessis M, Mohandas N and feo C. Automated ektacytometry: a new method of measuring red cell deformability and red cell indices. Blood Cells 6: 315-327, 1980.
Rose HG and Oklander M. Improved method for the extraction lipids from human erythrocytes. J. Lipid Res 6: 428-431, 1965.
Reed CP, Swisher SN, Marionetti GV, Eden EG. Studies of the lipids of the erythrocyte I. Quantitative analysis of the lipids of normal human red cells. J Lab. Clin. Med. 56: 281-289, 1960.
Winterbourne CC, Batt RD., Lipid composition of human red cells of different ages. Biochem. Biophys. Acta. 202: 1-8, 1970.
Kim YR and Ornstein L. Isovolumetric sphering of erythrocytes for more accurate and precise cell volume measurement by flow cytometry. cytometry 3: 419-427, 1983.
Tycko DH, Metz MH, Epstein EA and Grinbaum A. Flow Cytometric light scattering measurement of red blood cell volume and hemoglobin concentration. Applied Optics 24: 1355-1365, 1985.
Mohandas N. Kim YR, tycko HD, Orlik J, Wyatt J and Groner W. Accurate and independent measurement of volume and hemoglobin concentration of individual red cells by laser scattering. Blood 68: 506-513, 1986.
Eber SW, Pekrun A, Neufeldt A, and Schroter, W. Prevalence of increased osmotic fragility of erythrocytes in German blood donors: screening using a modified glycerol lysis test. Ann. Hamatol. 65:m 88-92, 1992.
Acharya J, Ferguson IL, Cassidy AG and Grimes AJ. An improved acidified glycerol lysis test (AGLT) used to detect spherocytosis in pregnancy. Br. J. Haematol 65: 343-345, 1987.
Bucx P, Breed WPM and Hoffman JJM. Comparison of acidified glycerol lysis test, pink test and osmotic fragility test in hereditaru spherocytosis: effect of incubation. Eur. J. Haematol 40: 277-231, 1988.
Gottfried EL, and Robertson NA. Glycerol lysis time as a screening test for erythrocyte disorders. J. Lab. Clin. Med. 84: 746-751, 1974.
Parpart AK, Lorenz PB, Parpart ER, Gregg JR and Chase A. The osmotic resistance (fragility) of human red cells. J. Clin. Invest. 26: 636-645, 1974.
Rutherford CJ. Postlewaight BF and Hallowes M. An evaluation of the acidified glycerol lysis test. Br. J. Haematol. 63: 119-121, 1986.
Vettore L, Zanella A. Molero GL, DeMatteis MC, Pavesi M, Mariani M. A new test for the laboratory diagnosis of spherocytosis. Acta Hematol. 72: 258-263, 1984.
Zanella A, Izzo C, Rebulla P, Zanuso F, Perroni L, and Sirchia G. Acidified glycerol lysis test: a screening test for spherocytosis. Br. J. Haematol. 45: 481-486, 1980.
Godal HC and Heisto H. High prevalence of increase osmotic fragility of red blood cells among Norwegian blood donors. Scand. J. Haematol. 27: 30-34, 1981.
Godal HC, Nyvold N and Rustad A. The osmotic fragility of red blood cells: a re-evaluation of technical conditions. Scand. J. Haematol 23: 55-58, 1979.
Godal HC Elde AT, Nyvold N and Brosstad F. The normal range of osmotic fragility of red blood cells. Scand. J. Haematol. 25: 107-112, 1980.
Fugawa N, Friedman S, Gill FM, Schwartz E and Shaller C. Hereditary spherocytosis with normal osmotic fragility after incubation. JAMA 242: 63-64, 1979.
Cynober T, Mohandas N and Tchernia G. Red cell abnormalities in hereditary spherocytosis-relevance to diagnosis and for understanding the variable depression of clinical severity. J.Lab. Clin. Med., 128: 259-269, 1996.
Bayer Corporation
Wallenhorst Maureen M.
LandOfFree
Methods and reagent compositions for the determination of membra does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and reagent compositions for the determination of membra, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and reagent compositions for the determination of membra will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-688564