Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1996-08-14
1999-09-21
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435193, 4353201, 536 232, C12N 1554, C12N 121, C12N 1563, C12N 910
Patent
active
059553472
ABSTRACT:
A method for isolating a gene, comprising:
(i) isolating a cell possessing a post-translational characteristic of interest, said post-translational characteristic being the presence of a membrane-bound oligosaccharide or polysaccharide of interest on the surface of said cell, the presence of a soluble oligosaccharide or polysaccharide of interest in an extract of said cell, or the presence of a particularly glycosyltransferase activity in an extract of said cell;
(ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of said isolated cell;
(iii) transforming host cells with said genetic library; and
(iv) screening said transformed host cells for a host cell containing said post-translational characteristic, thereby obtaining a cell containing said gene, is disclosed. The method can be used to obtain genes encoding glycosyltransferases.
REFERENCES:
patent: 4205129 (1980-05-01), Podolsky et al.
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4438200 (1984-03-01), Taubman et al.
patent: 4593004 (1986-06-01), Boross et al.
patent: 4634665 (1987-01-01), Axel et al.
patent: 4745055 (1988-05-01), Schenk et al.
patent: 5324663 (1994-06-01), Lowe
Lowe et al, FASEB Abstract Form, "Blood Group H .alpha.-2-L-Fucosyltransferase Expressed in Mouse Cells After Stable Transfection with Human DNA", (1988).
Kukowska-Latallo et al, (Sep. 22, 1988 Submission to UCLA Glycobiology Symposium).
Ernst et al, (Sep. 22, 1988 Submission to UCLA Glycobiology Symposium).
Lowe et al, (Sep. 22, 1988 Submission to UCLA Glycobiology Symposium).
Ernst et al, J. Biol. Chem., "Stable Expression of Blood Group H Determinants and GDP-L-fucose:.beta.-D-Galactoside 2-.alpha.-L-Fucosyltransferase in Mouse Cells after Transfection with Human DNA", (1989) 264, pp. 3436-3447.
Rajan et al, J. Biol. Chem., "A Cloned Human DNA Restriction Fragment Determines Expression of GD P-L-Fucose:.beta.-D-Galactoside 2-.alpha.-L-fucosyltransferase in Transfected Cells", (1989) 264, pp. 11158-11167.
Larsen et al, Proc. Natl. Acad. Sci., "Molecular Cloning, Sequence and Expression of a Human GDP-L-fucose:.beta.-D-galactoside 2-.alpha.-L-fucosyltransferase cDNA that can Form the H Blood Group Antigen", (1990) 87, pp. 6674-6678.
Larsen et al, Proc. Natl. Acad. Sci., "Isolation of a cDNA Encoding a Murine UDPgalactose:.beta.-D-galactosyl-1,4-N-acetyl-D-glucosaminide .alpha.-1,3-galactosyltransferase: Expression Cloning by Gene Transfer", (1989) 86, pp. 8227-8231.
Graham et al, Virology, "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA", (1973) 52, pp. 456-467.
Kukowska-Latallo et al, Genes & Devel., "A Cloned Human cDNA determines expression of a Mouse Stage-Specific Embryonic Antigen and the Lewis Blood Group .alpha.(1,3/1,4)fucosyltransferase", (1990) 4, pp. 1288-1303.
Weber et al, Mol. Cell. Biol., "Molecular Cloning and Biological Characterization of a Human Gene, ERCC2, That Corrects the Nucleotide Excision Repair Defect in CHO UV5 Cells", (1988) 8, pp. 1137-1146.
Ripka et al, Biochem. & Biophys. Res. Comm., "DNA-Mediated Transformation of N-Acetylglucosaminyltransferase I Activity into an Enzyme Deficient Cell Line", (1989), 159, pp. 554-560.
Ripka et al, (preprint) "Co-transformation of Lec1 CHO Cells with N-Acetylgucosaminyltransferase I Activity and a Selectable Marker".
Chao et al, Science, "Gene Transfer and Molecular Cloning of the Human NGF Receptor", (1986), 232, pp. 518-521.
Kavathas et al, Proc. Natl. Acad. Sci., "Stable Transformation of Mouse L Cells for Human Membrane T-Cell Differentiation Antigens, HLA and .beta..sub.2 -microglobulin: Selection by Fluorescence-activated Cell Sorting", (1983), 80, pp. 524-528.
Southern et al, J. Mol. Appl. Genet., "Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter", (1982), 1, pp. 327-341.
Kuhn et al, Cell, "Gene Transfer, Expression, and Molecular Cloning of the Human Transferrin Receptor Gene", (1984), 37, pp. 95-103.
Wigler et al, Cell, "Biochemical Transfer of Single-Copy Eucaryotic Genes Using Total Cellular DNA as Donor", (1978), 14, pp. 725-731.
Littman et al, Cell, "The Isolation and Sequence of the Gene Encoding T8: A Molecule Defining Functional Classes of T Lymphocytes", (1985), 40, pp. 237-246.
Toone et al, Tetrahedron Rep., (1989) "Enzyme-Catalyzed Synthesis of Carbohydrates" pp. 5365-5422.
Hodgson, Bio/Techn., "Capitalizing in Carbohydrates", (1990), 8, pp. 83-111 (partial).
Stinson, C&EN, "Controlled Linkage Techniques for Monosaccharides Introduced", (1989), Aug. 28, 1989, pp. 25-26.
C&EN, "Yale Carbohydrate Technology Licensed", (1990), Jan. 15, 1990, pp. 7-8.
Shaper et al, Proc. Natl. Acad. Sci., "Bovine Galactosyltransferase: Identification of a Clone by Direct Immunological Screening of a cDNA Expression Library", (1986), 83, pp. 1573-1577.
Appert et al, Biochem. Biophys. Res. Comm., "Isolation of a cDNA Coding for Human Galactosyltransferase", (1986), 139, pp. 163-168.
Narimatsu et al, Proc. Natl. Acad. Sci, "Cloning and Sequencing of cDNA of Bovine N-acetylglucosamine (.beta.1-4)galactosyltransferase", (1986), 83, 4720-4724.
Prieels et al, J. Biol. Chem., "Purification of the Lewis Blood Group N-Acetylglucosaminide .alpha.1-4 Fucosyltransferase and an N-Acetylglucosaminide .alpha.1-3 Fucosyltransferase from Human Milk", (1981) 256, pp. 10456-10463.
Eppenberger-Castori et al, Glycoconjugate J, "Purification of the N-Acetylglucosaminide .alpha.(1-3/4) Fucosyltransferase of Human Milk", (1989), 6, pp. 101-114.
Elices et al, J. Biol. Chem., "Purification and Characterization of UDP-Gal".beta.-D-Gal(1,4)-D-GlcNAc .alpha.(1,3)-Galactosyltransferase from Ehrlich Ascites Tumor Cells", (1986) 261, pp. 6064-6072.
Blanken et al, J. Biol. Chem., "Biosynthesis of Terminal Gal.alpha.1-3Gal.beta.1-4GlcNAc-R Oligosaccharide Sequences on Glyconconjugates", (1985), 260, pp. 12927-12934.
Beyer et al, J. Biol. Chem., "Purification to Homogeneity of the H Blood Gropu .beta.-Galactoside .alpha.1-2 Fucosyltransferase from Porcine Submaxillary Gland", (1980), 255, pp. 5364-5372.
Beyer et al, J. Biol. Chem., "Enzymatic Properties of the .beta.-Galactoside .alpha.1-2 Fucosyltransferase from Porcine Submaxillary Gland", (1980), 255, pp. 5373-5379.
Weinstein et al, J. Biol. Chem., "Primary Structure of .beta.-Galactoside .alpha.2,6-Sialyltransferase", (1987), 262, pp. 17735-17743.
Weinstein et al, J. Biol. Chem., "Purification of a Gal.beta.1-4GlcNAc .alpha.2-6 Sialyltransferase and a Gal.beta.1-3(4)GlcNAc .alpha.2-3 Sialyltransferase to Homogeneity from Rat Liver", (1982), 257, pp. 13835-13844.
Creeger et al, Meth. Enz., "Complex Carbohydrates", (1982), 83, pp. 326-331 (ed. V. Ginsborg).
Natsuka et al, J. Biol. Chem., "Molecular Cloning of a cDNA Encoding a Novel Human Leukocyte .alpha.-1,3-Fucosyltransferase Capable of Synthesising the Sialyl Lewis x Determinant", (1994), 269(24), pp. 16789-16794.
Natsuka et al, J. Biol. Chem., (Additions and Corrections), "Molecular Cloning of a cDNA Encoding a Novel Human Leukocyte .alpha.-1,3-Fucosyltransferase Capable of Synthesising the Sialyl Lewis x Determinant", (1994), 269(32), p. 20806.
Sasaki et al, J. Biol. Chem., "Expression Cloning of a Novel .alpha.-1,3-Fucosyltransferase that is Involved in Biosynthesis of the Sialyl Lewis x Carbohydrate Determinant in Leukocytes", (1994), 269(20), pp. 14730-14737.
Sarnesto et al, J. Biol. Chem., "Purification of the .beta.-N-Acetylglucosaminide .alpha.1-3-Fucosyltransferase from Human Serum", (1992),267, pp. 2745-2752.
Mollicone et al, Eur. J. Biochem., "Acceptor Specificity and Tissue Distribution of Three Human .alpha.-3-fucosyltransferases", (1990), 191, pp. 169-176.
Holmes et al, J. Biol. Chem., "Biosynthesis of the Sialyl-Le.sup.x Determinant Carried by Type 2 Chain Glycosphingolipids (IV.sup.3 NeuAcIII.sup.3 FucnLe.sub.4, VI.sup.3 NEuAcV.sup.3 FucnLc.sub.6, and VI.sup.3 NeuAcIII.sup.3 V.sup.3 Fuc.sub.2 nLc.sub.6) in Human Lung Carcinoma PC9 Cells", (1986), 261, pp. 3737-3743.
Weston et al, J. Cell. Biochem, "Defining a Glycosyltransferase Gene Family: Cloning and
Prouty Rebecca E.
The Regents of the University of Michigan
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