Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-06-06
1998-11-17
Railey, Johnny
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
424 932, 4353201, 514 44, 536 231, 536 232, 536 235, 536 2353, 536 236, 536 237, 536 2372, 536 241, 536 245, C12N 1509, C12N 1511, C12N 1563, C12N 1586
Patent
active
058375104
ABSTRACT:
Host cells may be treated for an infection or a hyperproliferative disorder which is characterized by the presence, in the affected cells, of a trans-acting factor capable of regulating gene expression by inserting into the cells a polynucleotide construct having a cis-acting regulatory sequence which is regulated by the trans-acting factor and an effector gene which renders said cell susceptible to protection or destruction. For example, the cis-acting region may be homologous to the HIV tar region, and the effector gene may encode ricin A or HSV-1 thymidine kinase. Upon infection with HIV, the HIV tat protein activates the tar region, and induces transcription and expression of ricin A, resulting in cell death, or of HSV-1 tk, resulting in cell death upon treatment with dideoxynucleoside agents such as acyclovir and gancyclovir.
REFERENCES:
patent: 4405712 (1983-09-01), Vande Woude et al.
patent: 4603112 (1986-07-01), Paoletti et al.
patent: 4650764 (1987-03-01), Temin et al.
patent: 4663281 (1987-05-01), Gillies et al.
patent: 4708818 (1987-11-01), Montanier et al.
patent: 4725669 (1988-02-01), Essex et al.
patent: 4738922 (1988-04-01), Haseltine et al.
patent: 4769330 (1988-09-01), Paoletti et al.
patent: 4861719 (1989-08-01), Miller
patent: 4868116 (1989-09-01), Morgan et al.
patent: 4980286 (1990-12-01), Morgan et al.
patent: 4980289 (1990-12-01), Temin et al.
patent: 5026635 (1991-06-01), Ferguson et al.
patent: 5091309 (1992-02-01), Schlesinger et al.
patent: 5192553 (1993-03-01), Boyse et al.
patent: 5246924 (1993-09-01), Fox et al.
patent: 5304489 (1994-04-01), Rosen
patent: 5306631 (1994-04-01), Harrison et al.
patent: 5399346 (1995-03-01), Anderson et al.
Adam et al., "Identification of a Signal in a Murine Retrovirus That is Sufficient for Packaging of Nonretroviral RNA Into Virions," J. Virology, 62:3802-3806 (1988).
Anderson, French W., "Human Gene Therapy," Science, 256:808-812 (1992).
Baltimore, David, "Intracellular Immunization," Nature, 335:395-396 (1988).
Cepko et al., "Construction and Applications of a Highly Transmissible Murine Retrovirus Shuttle Vector," Cell, 37:1053-1062 (1984).
Cone, et al., "Regulated Expression of a Complete Human .beta.-Globin Gene Encoded by a Transmissible Retrovirus Vector," Mol. Cell. Biol. 7: 887-897 (1987).
Cournoyer, Denis, Gene Therapy Of the Immune System, Annu. Rev. Immunology 11:297-329 (Apr. 12, 1993).
Danos, et al., "Safe and Efficient Generation of Recombinant Retroviruses With Amphotropic and Ecotropic Host Ranges," PNAS (USA), 85:6460-6464. (1988).
Dayton, et al., "The Trans-Activator Gene of the Human T Cell Lymphotropic Virus Type III Is Requested for Replication," Cell, 44:941-947 (1986).
Dzierzak, et al., "Lineage-Specific Expression of a Human .beta.-Globin Gene In Murine Bone Marrow Transplant Recipients Reconstituted With Retrovirus-Transduced Stem Cells," Nature, 331:35-41 (1988).
Felber, et al., "A Quantitative Bioassay for HIV-1 Based On Trans-Activation," Science, 239:184-187 (1988).
Felgner, et al., "Lipofection: A Highly Efficient, Lipid-Mediated DNA-Transfection Procedure," PNAS (USA), 84:7413 (1987).
Frankel, et al., "Dimerization of the tat Protein From Human Immunodeficiency Virus: A Cysteine-Rich Peptide Mimics The Normal Metal-Linked Dimer Interface," PNAS (USA), 85:6297-6300 (1988).
Frankel, et al., "Tat Protein From Human Immunodeficiency Virus Forms A Metal-Linked Dimer," Science, 240:70-73 (1988).
Friedman, et al., "Expresssion of a Truncated Viral Trans-Activator Selectively Impedes Lytic Infection by its Cognate Virus," Nature, 335:452-454 (1988).
Furman, et al., "Inhibition of Herpes Simplex Virus-Induced DNA Polymerase Activity and Viral DNA Replication by 9-(2-Hydroxyethoxymethyl)Guanine and Its Triphosphate," J. Virology, 32:72-77 (1979).
Ganz, et al., "Natural Peptide Antibiotics of Human Neutrophils," J. Clin. Invest. 76:1427-1435 (1985).
Goelz, "Hypomethylation of DNA From Benign and Malignant Human Colon Neoplasms," Science, 228:187-190 (1985).
Graham, et al., "A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA," Virology, 52:456-467 (1973).
Guild, et al., "Development of Retrovirus Vectors Useful for Expressing Genes in Cultured Murine Embryonal Cells and Hematopoietic Cells In Vivo," J. Virol. 62:3795-3801 (1988).
Haynes, Barton F., "Scientific and Social Issues of Human Immunodeficiency Virus Vaccine Development," Science, 260:1279-1286 (1993).
Hirsch, Martin S., "Aids Commentary: Azidothymidine," J. Infect. Dis., 157:427-431 (1988).
Ho, et al., "A T-Cell-Specific Transcriptional Enhancer Element 3' C.sub..varies. In The Human T-Cell Receptor .varies. Locus," PNAS (USA) 86:6714-6718 (1989).
Johnston, et al., "Present Status and Future Prospects For HIV Therapies," Science 260:1286-1293 (1993).
Kantoff, et al., "Correction of Adenosine Deaminase Deficiency In Cultured Human T and B Cells By Retrovirus-Mediated Gene Transfer," PNAS (USA), 83:6563-6567 (1986).
Kriegler, et al., "Transformation Mediated By the SV40 T Antigens: Separation of the Overlapping SV 40 Early Genes With A Retroviral Vector," Cell, 38: 483-491 (1984).
Malim, et al., "The HIV-1 rev Trans-Activator Acts Through A Structured Target Sequence To Activate Nuclear Export Of Unspliced Viral mRNA," Nature, 338:254-257 (1989).
Maniatis, et al., "Molecular Cloning: A Laboratory Manual," Cold Spring Harbor Press, Cold Spring Harbor, N.Y., at pp. 22-26 (1982).
Maniatis, et al., "Regulation of Inducible And Tissue-Specific Gene Expression," Science, 236: 1237-1245 (1987).
Mansour, et al., "Disruption of the Proto-Oncogene int-2 In Mouse Embryo-Derived Stem Cells: A General Strategy For Targeting Mutations to Non-Selectable Genes," Nature, 336:348-352 (1988).
Mariman, E.C.M., "New Strategies For AIDS Therapy and Prophylaxis," Nature, 318:414 (1985).
Miller, et al., "Redesign of Retrovirus Packaging Cell Lines To Avoid Recombination Leading to Helper Virus Production," Molecular and Cellular Biology, 6:2895-2902 (1986).
Mitsuya, et al., "Strategies For Antiviral Therapy In AIDS," Nature, 325:773-778 (1987).
Moolten, F.L., "Medical Hypotheses," Medical Hypotheses, 24:43-51 (1987).
Muesing, et al., "Regulation of mRNA Accumulation by a Human Immunodeficiency Virus Trans-Activator Protein," Cell, 48:691-701 (1987).
Nabel, et al., "Alternative Mechanisms for Activation of Human Immunodeficiency Virus Enhancer in T Cells," Science, 239:1299-1302 (1988).
Overhauser, et al., "Generation of Glucocorticoid-Responsive Moloney Murine Leukemia Virus By Insertion of Regulatory Sequences from Murine Mammary Tumor Virus Into The Long Terminal Repeat," J. of Virology, 54:133-144 (1985).
Palmiter, et al., "Cell Lineage Ablation In Transgenic Mice By Cell-Specific Expression Of A Toxin Gene," Cell, 80:435-443 (1987).
Patarca, et al., "rpt-1, An Intracellular Protein From Helper/Inducer T cells that Regulates Gene Expression Of Interleukin 2 Receptor And Human Immunodeficiency Virus Type 1," Proc. Natl. Acad. Sci. U.S.A., 85:2733-2737, (1988).
Peterlin, et al., "Elevated Levels of mRNA Can Account For The Trans-Activation of Human Immunodeficiency Virus," PNAS (USA), 83:9734 (1986).
Phelps, et al., "The Human Papillomavirus Type 16 E7 Gene Encodes Transactivation and Transformation Functions Similar To Those of Adenovirus E1A," Cell, 53:539-547 (1988).
Piatak, et al., "Expression of Soluble and Fully Functional Ricin A Chain in Escherichia Coli Is Temperature-Sensitive," The Journal of Biological Chemistry, 263 No. 10, 4837-4843 (1988).
Selsted, et al., "Primary Structures of Three Human Neutrophil Defensins," J. Clin. Invest., 76:1436-1439 (1985).
Shinnick, et al., "Nucleotide Sequence of Moloney Murine Leukaemia Virus," Nature, 293:543-548 (1981).
Smith, et al., "Blocking of HIV-1 Infectivity By a Soluble, Secreted Form of the CD4 Antigen," Science, 238:1704-1707 (1987).
Sodroski, et al., "Trans-Acting Transcriptional Regulation of Human T-Cell Leukemia Virus Type III Long Terminal Repeat," Science, 227:171-173 (1985).
Sodroski, et al., "Location Of the Trans-Activating Region On The Genome Of Human T-Cell Lymphotropi
Goldsmith Mark A.
Ralston Robert O.
Blackburn Robert P.
Kruse Norman J.
Pochopien Donald J.
Railey Johnny
LandOfFree
Methods and polynucleotide constructs for treating host cells fo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and polynucleotide constructs for treating host cells fo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and polynucleotide constructs for treating host cells fo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-883313