Methods and means of histone methylation

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C530S358000, C530S388100

Reexamination Certificate

active

07074578

ABSTRACT:
This invention relates to the methylation of histones, in particular to a previously uncharacterised group of histone H3 methylases which comprise a SET domain and which methylate either lysine 4 within the amino tail of histone H3 or within the histone H3 core. Methylation by these methylases, in particular trimethylation, is shown to be important for transcriptional activity.

REFERENCES:
patent: 2004/0053848 (2004-03-01), Allis et al.
patent: 1 162 274 (2001-12-01), None
patent: 1 162 460 (2001-12-01), None
patent: WO 02/18418 (2002-03-01), None
Strahl et al, PNAS, 1999, vol. 96, No. 26, pp. 14967-14972.
Allis et al, U.S. Appl. No. 60/227,757, filed Aug. 25, 2000.
Rea et al; “Regulation of Chromatin Structure by Site-Specific Histone H3 Methyltransferases”; Nature, Macmillan Journal Ltd., London, GB, vol. 406, No. 6796, Aug. 2000, pp. 593-599, XP002154907.
Nislow et al; “Set1, a Yeast Member of the Trithorax Family, Functions in Transcriptional Silencing and Diverse Cellular Process”; Molecular Biology of the Cell, vol. 8, No. 12, Dec. 1997, pp. 2421-2436, XP002248137.
Nagase et al; “Prediction of the Coding Sequences of Unidentified Human Genes VII. The Complete Sequences of 100 New CDNA Clones From Brain Whichcan Code for Large Proteins In Vitro”; DNA Research, Universal Academy Press, JP, vol. 4, No. 2, 1997, pp. 141-150, XP001052821.
Kikuno et al: “Prediction of the Coding Sequences of Unidentified Human Genes. XIV. The Complete Sequences of 100 New Large Proteins In Vitro”; DNA Research, Universal Academy Press, JP, vol. 6, 1999, pp. 197-205, XP000852618.
Santos-Rosa et al; “Active Genes are Tri-Methylated at K4 of Histone H3”; Nature (London), vol. 419,No. 6905, 2002, pp. 407-411, XP002248138.

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