Methods and means of determining microorganism population

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 40, 435292, 435301, C12Q 106, C12Q 108, C12M 126, C12M 120

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active

045872139

ABSTRACT:
The described method of determining viable microorganism population comprises spreading an experimentally pre-determined volume of an aqueous suspension of said microorganism, (such as bacteria, ricketsia, yeasts, fungi, molds, algae, viruses, protozes and similar organism), on the surface of a dried hydrophilic polymer film, capable of forming a solid (non-flowing) gel on rehydration and supported by a suitable (for subsequent operations) piece of material, (made of glass, ceramic, metal, plastic etc.), adaptable for coating, drying, and examining, said hydrophilic film with or without nutrient for said microorganisms and optionally containing a coloring matter capable to discolor, when in the vicinity of said viable microorganisms, which film by imbibing free water from the said suspension, functions as a filter to retain and distribute the microorganism content as a deposit on the surface of said film, (with or without natural nutrient present in said suspension), then incubating the microorganism deposit, whereby said microorganisms grow in colonies and determining the microorganism population by counting said colonies, or, if said coloring matter was included in said film, then determining said microorganism population measuring the time period during which said ratios of said coloring matter appears or by measuring the discoloration degree of said coloring matter in transmitted or reflected light, or alternatively counting said discolored colonies.

REFERENCES:
patent: 2672431 (1954-03-01), Goetz
patent: 2677646 (1954-05-01), Lovell et al.
patent: 2677647 (1954-05-01), Lovell
patent: 2761813 (1956-09-01), Goetz
Gershenfeld, Bacteriology and Allied Subject, pp. 77 to 79.
Abbott, Principles of Bacteriology 2nd Ed., pp. 68-72 and 101-110 (1894).

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