Methods and means for obtaining modified phenotypes

Details Methods and means for obtaining modified phenotypes Methods and means for obtaining modified phenotypes

2006-11-21

2006-11-21

Mehta, Ashwin (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide encodes an inhibitory rna molecule

C800S287000, C435S320100, C435S419000, C435S468000

Reexamination Certificate

active

07138565

ABSTRACT:
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.

REFERENCES:
patent: 5034323 (1991-07-01), Jorgensen et al.
patent: 5190931 (1993-03-01), Inouye
patent: 5231020 (1993-07-01), Jorgensen et al.
patent: 5283184 (1994-02-01), Jorgensen et al.
patent: 5908779 (1999-06-01), Carmichael et al.
patent: 0223399 (1987-05-01), None
patent: 0240208 (1987-10-01), None
patent: 0467349 (1992-01-01), None
patent: 0647715 (1995-04-01), None
patent: WO89/05852 (1989-06-01), None
patent: WO95/15394 (1995-06-01), None
patent: WO95/34668 (1995-12-01), None
patent: WO98/53083 (1998-11-01), None
patent: WO99/53050 (1999-10-01), None
Thomas et al., Plant Journal, 2001, vol. 25, pp. 417-425.
J.A. Brussian et al., “An Arabidopsis Mutant with a Reduced Level ofcab140RNA is a Result of Cosuppression”, The Plant Cell, vol. 5, Jun. 1993, p. 667-677, American Society of Plant Physiologists, Rockville, MD, USA.
W.G. Dougherty et al., “Transgenes and Gene Suppression: telling us something new?”, Current Opinion in Cell Biology, 1995, vol. 7, p. 399-405; Current Biology, London, UK.
M. Faske et al., “Transgenic Tobacco Plants Expressing Pea ChloroplastNmdhcDNA in Sense and Antisense Orientation”, Plant Physiol., 1997, vol. 115, p. 705-715, Am. Soc. Of Plant Physiologists, Lancaster, PA.
M.W. Graham et al., “Co-suppression, Anti-sense and Synthetic Viral Resistance: a Common Mechanism!”, Symposium 4-3, Abstract for talk given by Michael Graham at the Lome Genome Conference, Victoria, Australia in Feb. 1996.
M. Katsuki et al, “Conversion of Normal Behavior to Shiverer by Myelin Basic Protein Antisense cDNA in Transgenic Mice”, Science, vol. 241, Jul. 29, 1988, p. 593-595, Am. Assn for the Advancement of Science, Washington, DC.
Y.H. Kook et al., “The Effect of Antisense Inhibition of Urokinase Receptor in Human Squamous Cell Carcinoma on Malignancy”, The EMBO Journal, vol. 13, No. 17, p. 3938-3991, 1994, Oxford University Press, Oxford, England.
J.A. Lindbo et al., “Virus-Mediated Reprogramming of Gene Expression in Plants”, Current Opinion in Plant Biology, vol. 4, p. 181-185, 2001, Elsevier Science Ltd., Amsterdam, Holland.
P. Meyer, “Understanding and Controlling Transgene Expression”, TIBTECH, Sep. 1995, vol. 13, p. 332-337, Elsevier Science, Amsterdam, Holland.
P. Meyer, “Homology-Dependent Gene Silencing in Plants”, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1996, vol. 47, p. 23-48. Annual Reviews, Inc., Palo Alto, California.
M.K. Montgomery et al., “RNA as a Target of Double-stranded RNA-mediated Genetic Interference inCaenorhabditis elegans” Proc. Natl. Acad. Sci. USA, vol. 95, Dec. 1998, p. 15502-15507, the National Academy of Sciences, Washington, D.C.
M.C. Moroni et al, “EGF-R Antisense RNA Blocks Expression of the Epidermal Growth Factor Receptor and Suppresses the Transforming Phenotype of a Human Carcinoma Cell Line”, The Journal of Biological Chemistry, vol. 267, No. 5, issue of Feb. 5, 1992, p. 2714-2722, American Society for Biochemistry and Molecular Biology, Baltimore, MD.
C. Napoli et al., “Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genesin trans”, the Plant Cell, vol. 2, Apr. 1990, p. 279-289, American Society of Plant Physiologists, Rockville, MD.
J.C. Sandford et al., “The Concept of Parasite-Derived Resistance—Deriving Resistance Genes from the Parasite's own Genome”, J. Theor. Biol., 1985, vol. 13, p. 395-405, Academic Press Inc., London, England.
K.W. Savin et al., “Antisense ACC Oxidase RNA Delays Carnation Petal Senescence”, HortScience, vol. 30(5), Aug. 1995, p. 970-972, HortScience is a publication of the American Society for Horticulture Science.
W. Schiebel et al., “RNA-directed RNA Polymerase from Tomato Leaves”, The Journal of Biological Chemistry, vol. 268, No. 16, Jun. 5, 1993, p. 11858-11867, American Society for Biochemistry and Molecular Biology, Inc., Baltimore, MD.
R.E. Sheehy et al., “Reduction of Polygalacturonase Activity in Tomato Fruit by Antisense RNA”, Proc Natl Acad. Sci, USA, vol. 85, Dec. 1988, p. 8805-8809, the National Academy of Sciences, Washington, D.C.
B.A. Sullenger et al, “Analysis oftran-Acting Response Decoy RNA-Mediated Inhibition of Human Immunodeficiency Virus Type 1 Transactivation”, Journal of Virology, Dec. 1991, vol. 65, No. 12, p. 6811-6816, American Society for Microbiology, Washington D.C.
S. Swaney et al., “RNA-Mediated Resistance with Nonstructural Genes from the Tobacco Etch Virus Genome”, MPMI vol. 8, No. 6, 1995, p. 1005-1011, The American Phytopathological Society, St. Paul, Minnesota.
R. Van Blokland et al, “Transgene-mediated Suppression of Chalcone Synthase Expression inPetunia hybridaResults from an increase in RNA Turnover”, The Plant Journal, 1994, vol. 6, No. 6, p. 861-877, Blackwell Sciences, Oxford, England.
A.R. van der Krol et al., “Flavanoid Genes in Petunia: Addition of a Limited Number of Gene Copies May Lead to a Suppression of Gene Expression”, The Plant Cell, vol. 2, Apr. 1990, p. 291-299, American Society of Plant Physiologists, Rockville, MD.
Marathe, “CIS-Repeat Induced Gene Silencing in Tobaco”, Ph.D. Thesis, University of South Carolina, 1997.
Bramlage et al., “Designing Ribozymes For the Inhibition of Gene Expression”,TIBTECH16:434-438 (1998), Barking Elsevier Science Publishers, Netherlands.
Covey et al., “Plants Combat Infection by Gene Silencing”,Nature385:781-782 (1997), Nature Publishing Group, Hampshire, United Kingdom.
Eckner et al. “Mature mRNA 3' End Formation Stimulates RNA Export From the Nucleus”,EMBO J. 10:3513-3522 (1991), Oxford University Press, Oxford, United Kingdom.
Egli and Braus, “Uncoupling of mRNA 3' Cleavage and Polyadenylation by Expression of a Hammerhead Ribozyme in Yeast”,J. Biol. Chem.269:27378-27383 (1994), American Society for Biochemistry and Molecular Biology, Bethesda, Maryland, USA.
Hamilton et al., “A Transgene With Repeated DNA Causes High Frequency, Post-Transcriptional Suppression of ACC-Oxidase Gene Expression in Tomato”,The Plant Journal15(6):737-746 (1998), Blackwell Sciences Ltd., Oxford, United Kingdom.
Haseloff et al., “Simple RNA Enzymes With New and Highly Specific Endoribonuclease Activities”,Nature334:585-591 (1988), Nature Publishing Group, Hampshire, UK.
Lee et al., “Post-Transcriptional Gene Silencing of ACC Synthase in Tomato Results From Cytoplasmic RNA Degradation” ,The Plant Journal12(5):1127-1137 (1997), Blackwell Sciences Ltd., Oxford, United Kingdom.
Mette et al., “Production of Aberrant Promoter Transcripts Contributes to Methylation and Silencing of Unlinked Homologous Promotersin trans”,The EMBO Journal18:241-248 (1999), Oxford University Press, Oxford, United Kingdom.
Liu, Zhong et al., “Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA”,Proc Nat Acad Sci USA, May 10, 1994, 91(10):4258-62, The National Academy of Sciences, U.S.A.
Donahue, C.P. et al., “Kinetics of hairpin ribozyme cleavage in yeast”,RNA, Sep. 1997, 3(9):961-73, Cambridge University Press, U.S.A.
Welch, P.J. et al., “Expression of ribozymes in gene transfer systems to modulate target RNA levels”,Curr Op Biotech, Oct. 1998, 9(5):486-96, Current Biolog

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