Methods and means for influencing intracellular...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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Reexamination Certificate

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07316913

ABSTRACT:
The invention relates to a method for the investigation of intercellular communication and intercellular transport, wherein after singularisation cells are investigated for membrane tubes which contain F-actin and myosin, have a diameter of 50 to 400 nm, as a rule are up to 50 micrometers long or in same cases longer, and which span between the cells. The invention further relates to a method wherein the organelle transport between the cells is investigated.

REFERENCES:
patent: WO 98/02186 (1998-01-01), None
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Rustom et al, “Analysis of Fast Dynamic Processes in Living Cells: High-Resolution and High-Speed Dual-Color Imaging Combined with Autmoated Image Analysis,” (BioTechniques), 2000, vol. 28, p. 722-726, 728, 730.
Yang et al, “Dual color microscopic imagery of cells expressing the green fluorescent protein and a red-shifted variant,” (Gene), 1996, vol. 173, p. 19-23.
Ramirez-Weber et al, “Cytonemes: Cellular Processes that Project to the Principal SIgnaling Center in Drosophila Imaginal Discs,” (Cell), 1999, vol. 97, p. 599-607.
Rudolf et al, “Dynamics of Immature Secretory Granules: Role of Cytoskeletal Elements during Transport, Cortical Restriction, and F-Actin-dependent Tethering,” (Molec. Biol.), 2001, vol. 12, p. 1353-1365.
Elliott G et al., Cell, Cell vol. 88, pp. 223-233, 1997.
Nathalie et al., Journal of Neuroscience Methods, vol. 73, No. 2, 1997, pp. 169-176.

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