Methods and means for controlling the stability of proteins

Details Methods and means for controlling the stability of proteins Methods and means for controlling the stability of proteins

1989-08-25

1994-03-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 4351721, 435191, 435192, 536 232, 935 10, 935 14, 935 23, C07H 2104, C12N 906, C12N 908, C12N 1553

Patent

active

052906906

ABSTRACT:
The invention pertains to a method for the production of a biologically active modified protein derived from a starting protein having essentially the same kind of biological activity with an attendant modulation effect on, particularly increase of, the stability as compared with that of the starting protein. The method comprises substituting an arginine residue for a lysine residue of the starting protein at a site that can sterically accommodate the substitution, without substantially altering the biological activity of the starting protein, said site being preferably of low solvent accessibility, at interfaces between domains or sub-units of the starting protein.

REFERENCES:
patent: 4410627 (1983-10-01), Lloyd et al.
patent: 4904584 (1990-02-01), Shaw
Shaw et al., Protein Engineering (Apr. 5-8, 1987) 1(3):264.
Rey et al., Biol Abstracts (1989) 87(6):AB-929, abstract No. 63577.
Argos, P. et al., "Thermal Stability and Protein Structure," Biochemistry 18(25):5698-5703 (1979).
Beyer, W. F. et al., "Examination of the Role of Arg-143 in Human Copper and Zinc Superoxide Dismutase by Site-Specific Mutagenesis." J. Biol. Chem. 262:11182-11187 (1987).

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