Methods and materials for restriction endonuclease applications

Details Methods and materials for restriction endonuclease applications Methods and materials for restriction endonuclease applications

1994-12-22

1997-02-18

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 4351721, 435810, 536 231, 536 2432, 536 2433, 536 253, 536 2532, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500

Patent

active

056040989

ABSTRACT:
The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.

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