Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-03-20
2007-03-20
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S455000
Reexamination Certificate
active
10404724
ABSTRACT:
The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.
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Ciafre, et al., “A Plasmid Family Containing Two Different Expression Cassettes Suitable for Immunomodulation and Genetic Immunization”Plasmid40:84-89 (1998) Article No. PL981339.
Papa, et al., “Development of a Multigenic Plasmid Vector for HCV DNA Immunization,”Res. Virol. 149:315-319 (1998).
Huynh, et al., Construction of Modular and Versatile Plasmid Vectors for the High-level Expression of Single or Multiple Genes in Insects and Insect Cell Lines,J. Mol. Biol288:13-20 (1999).
New, et al., “Co-expression of two gene products in the CNS using double-cassette defective herpes simplex virus vectors,”Molecular Brain Research 37:317-323 (1996).
PCT International Search Report, Application No. PCT/US03/10154 dated Aug. 21, 2003 (4 pages).
Dollard Anne
Ketter James
McNicholas Janet
Polo John M.
XOMA Technology Ltd.
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