Methods and materials for expression of human plasminogen varian

Details Methods and materials for expression of human plasminogen varian Methods and materials for expression of human plasminogen varian

1991-08-29

1993-03-02

Wax, Robert A.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435217, 435216, 435226, 514822, C12N 1558, C12N 968, A61K 37547, A61K 3762

Patent

active

051907569

ABSTRACT:
A cleavage-resistant plasminogen molecule is provided that is conveniently produced in recombinant cells by expression of a nucleic acid sequence encoding the plasminogen molecule. Preferably the plasminogen is a sequence variant with a modification in its two-chain cleavage site. The plasminogen molecule may be purified, acylated, complexed with acylated or non-acylated fibrinolytic enzymes, and formulated into pharmaceutical compositions for use in thrombolytic therapy.

REFERENCES:
patent: 3943245 (1976-03-01), Silverstein
patent: 5087572 (1992-02-01), Castellino
Davidson et al., Biochemistry 29:3585 (1990).
McClintock et al., Biochem. Biophys. Res. Comm., 43(3):694 (1971).
Whitefleet-Smith et al., Arch. Biochem. Biophys., 271, 390-399 (1989).
Forsgren et al., FEBS Letts, 213, 254-260 (1987).
Malinowski et al., Biochem., 23, 4243-4250 (1984).
McLean et al., Nature, 330, 132-137 (1987).
Wiman, Eur. J. Biochem., 39, 1-9 (1973).
Castellino & Powell, Methods of Enzymology, 80:365-379 (1981).
Sottrup-Jensen et al., Prog. Chem. Fibrinolysis Thrombolysis, 3:191-209 (1978).
Wiman, Eur. J. Biochem., 76:129-137 (1977).
Violand & Castellino, J. Biol. Chem., 251:3906-3912 (1976).
Castellino, Bioscience, 33:647-650 (1983).
Anderle et al., Haemostasis, 18:(Suppl. 1), 165-175 (1988).
Busby et al., Fibrinolysis, 2:64 (1988) (Suppl. I).
Bajaj & Castellino, J. Biol. Chem., 252:492-498 (1977).

Affiliated with

Also associated with

Rating

Methods and materials for expression of human plasminogen varian does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and materials for expression of human plasminogen varian, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and materials for expression of human plasminogen varian will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-125436