Methods and materials for enhanced somatic embryo regeneration i

Details Methods and materials for enhanced somatic embryo regeneration i Methods and materials for enhanced somatic embryo regeneration i

1986-07-21

1989-04-04

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352405, 43524051, 43524054, C12N 500, C12N 502

Patent

active

048186937

ABSTRACT:
Methods and compositions are provided to improve the quantity of embryos obtained by induction of somatic plant tissue. These involve the addition of effective amounts of selected amino acids, their derivatives or combinations of these as a source of reduced nitrogen, together with an optimum auxin dosage to the nutritive media used to regenerate or maintain the embryonic tissue. A synergistic effect is found by adding these amino acids either in combination or in combination with ammonium ion together with the selected auxin dosage.

REFERENCES:
Tazawa et al., 1969, Protoplasma 68: 157-173.
Walker et al., 1981, Plant Cell Tiss. Org. Cult. 1: 109-121.
Green et al., 1983, Miami Wint. Symp. 20: 147-157.
Wetherell et al., 1976, Physiol. Plant. 37: 97-103.
The Fifteenth Miami Winter Symposium, 17-21 Jan. 1983, Konover Hotel, Miami Beach, Fla., "Advances in Gene Technology: Molecular Genetics of Plants and Animals".

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