Methods and kits for reducing non-specific nucleic acid...

Details Methods and kits for reducing non-specific nucleic acid... Methods and kits for reducing non-specific nucleic acid...

2011-08-09

2011-08-09

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200

Reexamination Certificate

active

07993839

ABSTRACT:
Methods and kits for efficient amplification of nucleic acids are provided. The methods comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having terminal mismatch primer-dimer structure. The methods also comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having nucleotide analogues. The methods enhance efficiency of nucleic acid amplification reaction by reducing non-specific amplification reactions.

REFERENCES:
patent: 2004/0209299 (2004-10-01), Pinter et al.
patent: 2006/0073511 (2006-04-01), Jones et al.
patent: 2006/0216724 (2006-09-01), Christians et al.

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