Methods and kits for proximity probing

Details Methods and kits for proximity probing Methods and kits for proximity probing

2007-12-11

2007-12-11

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024310, C536S024320, C530S350000

Reexamination Certificate

active

09785657

ABSTRACT:
The present invention relates to sensitive, rapid and convenient assays for detection and/or quantification of one or several analyte(s) in solution using so called proximity probes. The proximity probes comprise a binding moiety and a nucleic acid. The nucleic acid from one proximity probe is only capable of interaction with the nucleic acid from the other proximity probe when these are in close proximity, i.e. have bound to the analytes for which they are specific. The present invention relates to methods and kits for proximity probing and are performed in solution without the need of a solid phase.

REFERENCES:
patent: 4988617 (1991-01-01), Landegren et al.
patent: 5871921 (1999-02-01), Landegren et al.
patent: 6306587 (2001-10-01), Royer et al.
patent: 6511809 (2003-01-01), Baez et al.
patent: 0 320 308 (1989-06-01), None
patent: WO 93/06240 (1993-04-01), None
patent: WO 97/00446 (1997-01-01), None
patent: WO 97/00446 (1997-01-01), None
patent: WO 9700446 (1997-01-01), None
patent: WO 9732044 (1997-09-01), None
Hart, Hiram and Elaine Greenwald, “Scintillation Proximity Assay (SPA)- A New Method of Immunoassay”, Molecular Immunology, vol. 16, pp. 265-267, 1979.
Szöllösi, János, et al., “Application Of Fluorescence Resonance Energy Transfer In The Clinical Laboratory: Routine And Research”, Cytometry (Communications in Clinical Cytometry), vol. 34, pp. 159-178, 1998.
Li, Fusheng, et al., “Homogeneous Noncompetitive Immunoassay Based on the Energy Transfer Between Fluorolabeled Antibody Variable Domains (Open Sandwich Fluoroimmunoassay)”, BIOTECHNIQUES, VOL. 27, No. 4, pp. 738-743, 1999.
Koo, Edward, et al., “Amyloid Diseases: Abnormal Protein Aggregation In Neurodegeneration”, Proc. Natl. Acad. Sci., vol. 96(18), pp. 9989-9990, Aug. 1999.
Prusiner, Stanley, et al. “Scrapie Prions Aggregate To Form Amyloid-Like Birefringent Rods”, CELL, vol. 35 (2 Pt 1), pp. 349-358, Dec. 1983.
Nadeau, James, et al. “Real-Time, Swequence-Specific Detection of Nucleic Acids During Strand Displacement Amplification”, Analytical Biochemistry, vol. 276, pp. 177-187, 1999.
van Deursen, Peter, et al., “A Novel Quantitative Multiplex NASBA Method: Application to Measuring Tissue Factor and CD14 mRNA Levels in Human Monocytes”, Nucleic Acids Research, vol. 27, No. 17, pp. I-vi, 1999.
White, Stephanie, and Theodore Christopoulos, “Signal Amplification System for DNA Hybridization Assays Based on In Vitro Expression of a DNA Label Encoding Apoaequorin”, Nucleic Acids Research, vol. 27, No. 19, pp. i-viii, 1999.
Kwiatkowski, Robert, et al., “Clinical, Genetic, and Pharmacogenetic Applications of Invader Assay”, Molecular Diagnosis, vol. 4, No. 4, pp. 353-364, 1999.
Gibson, Ursula, et al. “A Novel Method for Real Time Quantitative RT-PCR”, Genome Research, vol. 6, pp. 995-1001, 1996.
Tyagi Sanjay and Fred Russell Kramer, “Molecular Beacons: Probes that Fluoresce upon Hybridization”, Nature Biotechnology, vol. 14, pp. 303-308, Mar. 1996.
Steuerwald, Nury, et al., “Analysis of Gene Expression in Single Oocyte and Embryos by Real-Time Rapid Cycle Fluorescence Monitored RT-PCR”, Molecular Human Reproduction, vol. 5, No. 11, pp. 1034-1039, 1999.
Gentalen, Erik and Mark Chee, “A Novel Method for Determining Linkage Between DNA Sequences: Hybridization to Paired Probe Arrays”, Nucleic Acids Research, vol. 27, No. 6, pp. 1485-1491, 1999.
Banér, Johan, et al. “Signal Amplification of Padlock Probes by Rolling Circle Replication”, Nucleic Acids Research, vol. 26, No. 22, pp. 5073-5078, 1998.
Nilsson, Mats, et al., “Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection”, SCIENCE, vol. 265, pp. 2085-2088, Sep. 30, 1994.
Gold, Larry, et al., “Diversity of Oligonucleotide Functions”, Annu. Rev. Biochem., vol. 64, pp. 761-797, 1995.
Green, Louis, et al., “Inhibitory DNA Ligands to Platelet-Derived Growth Factor B-Chain”, BIOCHEMISTRY, vol. 35, pp. 14413-14424, 1996.

Affiliated with

Also associated with

Rating

Methods and kits for proximity probing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and kits for proximity probing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and kits for proximity probing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3853836