Methods and kits for obtaining DNA end sequence information...

Details Methods and kits for obtaining DNA end sequence information... Methods and kits for obtaining DNA end sequence information...

2005-09-13

2005-09-13

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100

Reexamination Certificate

active

06942975

ABSTRACT:
The present invention provides a method of producing a cloned insert that is representative of the ends of a large segment of DNA from the genome of an organism. Specifically, the present invention provides a clone insert strategy that comprising the steps of:a) isolating a nucleic acid molecule, or portion thereof, wherein said nucleic acid molecule is at least 20 kb;b) ligating said nucleic acid molecule into a vector molecule;c) cutting said nucleic acid molecule with a restriction endonuclease, wherein said restriction endonuclease cuts said isolated nucleic acid molecule of step (a) in at least two sites but does not cut said vector DNA,d) ligating said cut nucleic acid molecule;e) transforming a host cell with said ligated nucleic acid molecule to propagate said molecule;f) determining the sequence of the ends of the nucleic acid insert and the sequence from the restriction endonculease site.

REFERENCES:
patent: 6475731 (2002-11-01), Holt et al.

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