Methods and kits for nucleic acid analysis using fluorescence re

Details Methods and kits for nucleic acid analysis using fluorescence re Methods and kits for nucleic acid analysis using fluorescence re

1996-12-17

1999-08-31

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 935 77, 935 78, C12Q 168

Patent

active

059452838

ABSTRACT:
A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

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