Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2002-02-05
2008-07-29
Qian, Celine (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100, C435S325000, C536S024100
Reexamination Certificate
active
07405081
ABSTRACT:
This invention relates to methods and kits for determining the level of H2O2inside a cell, and for determining whether a test compound has ability to scavenge a reactive oxygen species (ROS). The methods and diagnostic kits of this invention employ a cell containing a promoter which is inducible by an ROS, such as the H2O2-inducible KatA promoter ofAgrobacterium tumefaciens. The methods of this invention may also be used to select for new or improved ROS scavengers by expressing a library of test scavengers in cells which express a reporter from a ROS-inducible promoter and selecting for those cells whose level of ROS-inducible expression of the reporter is reduced.
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