Methods and kits for detecting viral reverse transcriptase activ

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 435 911, 435 912, 536 231, 536 2433, C12Q 170, C12Q 168, C12P 1934, C07H 2102

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active

058174575

ABSTRACT:
Improved assay for the detection of viral reverse transcriptase activity using PCR conducts the reverse transcription reaction at an acidic pH or elevated temperature. The assay is useful in testing a wide variety of biological products and tissue samples.

REFERENCES:
patent: 5187060 (1993-02-01), Cerutti et al.
patent: 5310652 (1994-05-01), Gelfand et al.
patent: 5407800 (1995-04-01), Gelfand et al.
Dorothy E. Schumm "Essentials of Biochemistry, F. A. Davis Company, Philadelphia" pp. 15-17, 1988.
Bradford, M.M. "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," Analytical Biochemistry (1976) 72:248-254.
Busso, M. et al., "Development of an assay that detects transcriptionally competent human immunodificiency virus type one particles," Journal of Virological Methods (1994) 470:129-140.
Cann, A.J. et al., "Human T-Cell Leukemia Virus Types I and II," Virology, 2nd Ed., ed. by B.N. Fields, D.M. Knipe et al. Raven Press, Ltd., New York .COPYRGT. 1990:1501-1528.
Coffin, J.M. "Retroviridae and Their Replication," Virology, 2nd Ed., ed by B.N. Fields, D.M. Knipe et al. Raven Press, Ltd., New York .COPYRGT. 1990:1437-1500.
Grigoryan, M.S. et al., "Activation of putative transposition intermediate formation in tumor cells," The EMBO Journal (1985) 4(9):2209-2215.
Heneine, W. et al., "Detection of Reverse Transcriptase by a Highly Sensitive Assay in Sera from Persons Infected with Human Immunodeficiency Virus Type 1," The Journal of Infection Diseases (1995) 171:1210-1216.
Hirsch, M.S. et al., "Human Immunodeficiency Virus Biology and Medical Aspects," Chapter 54, Virology, 2nd Ed., ed. by B.N. Fields, D.M. Knipe et al. Raven Press, Ltd., New York .COPYRGT. 1990:1545-1570.
Innis, M.A. et al., PCR Protocols--A Guide to Methods and Applications, Academic Press (1990) (Table of Contents only).
Kornberg, A. et al., DNA Replication, 2nd Ed., W.H. Freeman and Company, New York (1992) p.151-152, p. 209.
Lisby, G. "Search for an HTLV-I-like retrovirus in patients with MS by enzymatic DNA amplification," Acta Neurol. Scand. (1993) 88:385-387.
Lugert, R. et al., "Specific Suppression of False-Positive Signals in the Product-Enhanced Reverse Transcriptase Assay," BioTechniques (Feb. 1996)20:210-217.
Losikoff, A.M. et al., "Industrial Experience with the Detection of Retroviruses," Develop. biol. Standard (19920 76:187-200.
Moore, W.A. "Experience in Cell Line Testing," Develop. biol. Standard (1992) 76:51-56.
Pyra, H et al., "Ulrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement," Proc. Natl. Acad. Sci. USA (Feb. 19940 91:1544-1548.
Reddy, E.P. et al., "Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients," Science (Jan. 1989) 243:529-533.
Roy-Burman, P. et al., "Assay For Type C Virus in Mouse Sera Based on Paticulate Reverse Transcriptase Activity," Journal of Virology (Sep. 1976) 19(3):1107-1110.
Silver, J. et al., "An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions," Nucleic Acids Research (1993) 21(15):3593-3594.

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