Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1994-09-26
1996-04-09
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 28, 435 25, 435810, 435 4, 435 792, 435 79, 435 78, C12Q 148, C12Q 126, G01N 3353
Patent
active
055061141
ABSTRACT:
Methods for detecting the presence or concentration of an analyte in a matrix by exploiting the specificities of the analyte's antibody and enzyme-substrate are disclosed. The analyte is first extracted from the matrix by binding it to its specific antibody which has been attached to a solid surface. The antibody-bound analyte is then separated from the matrix to remove it from any interfering substances. Next, the antibody-bound analyte is reacted with its specific enzyme-substrate to generate a signal proportionate to the amount of analyte bound to the antibody, which in turn is dependent on the quantity of analyte present in the matrix. Applications of the methods of the present invention to detect the presence and concentration of hemoglobin, a transferase, and a serine protease, are specifically disclosed. Further, kits useful for utilizing the methods of the present invention are also disclosed.
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Leary Louise N.
Osborn Laboratories
Reiter Stephen E.
Wityshyn Michael G.
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