Methods and kits for detecting protein kinases

Details Methods and kits for detecting protein kinases Methods and kits for detecting protein kinases

2005-06-28

2005-06-28

Leary, Louise N. (Department: 1654)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S021000, C435S004000, C435S008000, C435S023000

Reexamination Certificate

active

06911319

ABSTRACT:
A method for measuring protein kinase activity comprising:(a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested;(b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a);(c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and(d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.

REFERENCES:
patent: 4235961 (1980-11-01), Lundin
patent: 4246340 (1981-01-01), Lundin et al.
patent: 4286057 (1981-08-01), Wulff et al.
patent: 5330906 (1994-07-01), Kajiyama et al.
patent: 5374534 (1994-12-01), Zomer et al.
patent: 5583024 (1996-12-01), McElroy et al.
patent: 5648232 (1997-07-01), Squirrell
patent: 5759795 (1998-06-01), Jubin
patent: 5925558 (1999-07-01), Tsien et al.
patent: 6074859 (2000-06-01), Hirokawa et al.
patent: 6310060 (2001-10-01), Barrett et al.
patent: 6503723 (2003-01-01), Van Lune et al.
patent: 6599711 (2003-07-01), Crouch et al.
patent: 2002/0172991 (2002-11-01), Crouch et al.
patent: 29 08 054 (1980-09-01), None
patent: 694 12 790 (1993-01-01), None
patent: 0 022 432 (1981-01-01), None
patent: 0 301 541 (1989-02-01), None
patent: 0 449 621 (1996-08-01), None
patent: 0 794 260 (1997-09-01), None
patent: 2055200 (1981-02-01), None
patent: 2323167 (1999-06-01), None
patent: WO 94/17202 (1994-08-01), None
patent: WO 95/18853 (1995-07-01), None
patent: WO 96/22376 (1996-07-01), None
patent: WO-98/09169 (1998-03-01), None
patent: WO 98/28437 (1998-07-01), None
patent: WO 98/46729 (1998-10-01), None
patent: WO 99/02697 (1999-01-01), None
patent: WO 99/37799 (1999-07-01), None
patent: WO 99/41408 (1999-08-01), None
patent: WO 00/70082 (2000-11-01), None
Sqirrell et al., Journal Defense Sciense, 2:229-297 (1997).
T. Halonen, et al., “Bioluminescent Assay of Total and Brain-Specific Creatine Kinase Activity in Cerebrospinal Fluid,” Journal of Neurochemistry, vol. 39, pp. 36-43 (Nov. 1, 1982).
Tecan, “A New Generic Luminescent Protein Kinase Assay Suitable for High Throughout Screening,” Ultra Evolution, application note released in cooperation with Cambrex, www.tecan.com.
R. Somberg, Ph.D., et al., “A Universal Kinase Assay for a World of Kinases,” Promega, ppg. 14-17, No. 83 2003, www.promega.com.
Molecular Probes, “ATP Determination Kit (A-6608),” ATP Determination Kit (revised: Jul. 22, 1999), with English translation of D5.
E.M. Golding et al., Adjustment of K'to varying pH and pMg for the creatine kinase, adenylate kinase and ATP hydrolysis equilibria permitting quantitative bioenergetic assessment, 1995, pp. 1775-1782, vol. 198, The Journal of Experimental Biology.
W.E. Teague, Jr. et al., Adjustment of K'for the creatine kinase, adenylate kinase and ATP hydrolysis equilibria to varying temperature and ionic strength, 1996, pp. 509-512, vol. 199, The Journal of Experimental Biology.
T. Decker et al., A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity, 1988, pp. 61-69, vol. 15, Journal of Immunological Methods.
M.M. Nociari et al, A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity, 1998, pp. 157-167, vol. 213, Journal of Immunological Methods.
M. Bachy et al., Beta galactosidase release as an alternative to chromium release in cytotoxic T-cell assays, 1999, pp. 37-46, vol. 230, Journal of Immunological Methods.
N. Kasatori et al., Cytotoxicity test based on luminescent assay of alkaline phosphatase released from target cells, 1994, pp. 1050-1054, vol. 42, Rinsho Buori.
S. D'Atri et al., A miniaturized cell-mediated cytotoxicity assay with human effector mononuclear cells, 1986, pp. 383-390, vol. 5, Int. J. Tiss. Reac. VIII.
G. Feutren et al., Immune lysis of hepatocytes in culture: accurate detection by aspartate aminotransferase release measurement, 1984, pp. 85-94, vol. 75, Journal of Immunological Methods.
J.G. Pastorino et al., Functional consequences of the sustained or transient activation by bex of the mitochondrial permeability transition pore*, Oct. 29, 1999, pp. 31734-31739, vol. 274, No. 44, The Journal of Biological Chemistry.
G. Sala-Newby et al., Engineering firefly luciferase as an indicator of cyclic AMP-dependent protein kinase in living cells, Jul. 1992, pp. 241-244, vol. 307, No. 2, FEBS 11346.
B.R. Branchini et al., The role of lysine 529, a conserved residue of the acyl-adenylate-forming enzyme superfamily, in firefly luciferase, 2000, pp. 5433-5440, vol. 39, No. 18, Biochemistry 2000.
B.R. Branchini et al., Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color, 1999, pp. 13223-13230, vol. 38, No. 40, Biochemistry.
P.J. White et al., Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354, 1996, pp. 343-350, vol. 319, Biochem. J.
J.H. Devine et al., Luciferase from the East European firefly luciola mingrelica: cloning and nucleotide sequence of the cDNA, overexpression inEscherichia coliand purification of the enzyme, 1993, pp. 121-132, vol. 1173, Biochimica et Biophysica Acta.
C.B. Cohen et al., A microchip-based enzyme assay from protein kinase A, 1999, pp. 89-97, vol. 273, Analytical Biochemistry.
J. Eu et al., Homogeneous bioluminescence assay for galactosuria: interference and kinetic analysis, 1999, pp. 168-176, vol. 271, Analytical Biochemistry.
C. Lehel et al., A chemiluminescent microtiter plate assay for sensitive detection of protein kinase activity, 1997, pp. 340-346, vol. 244, Analytical Biochemistry.
H. Tatsumi et al., Construction of biotinylated firefly luciferases using biotin acceptor peptides, 1996, pp. 176-180, vol. 243, Analytical Biochemistry.
A. Thore, Technical aspects of the bioluminescent firefly luciferase assay of ATP, 1979, pp. 30-34, vol. 26, No. 2, Science Tools.
T. Olsson et al., Leakage of adenylate kinase from stored blood cells, 1983, pp. 437-445, vol. 5, Journal of Applied Biochemistry.
M. Karp et; al., A streptavidin-luciferase fusion protein: comparisons and applications, 1999, pp. 101-104, vol. 16, Biomolecular Engineering.
L. Chinsoo Cho, To lap or not to lap, 1996, pp. 120-124, vol. 1, The Oncologist.
Karamohamed et al., “Real-time Bioluminometric Method for Detection of Nucleoside Diphosphate Kinase Activity”,Biotechniques, 26:728-734 (1999).
Handa et al., “Assay of Adenosine 3', 5' Cyclic Monophosphate by Stimulation of Protein Kinase: A Method Not Involving Radioactivity”,Analytical Biochemistry, 102:332-339 (1980).

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