Methods and kits comprising AFLP primers, and ramp primers...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200

Reexamination Certificate

active

10276401

ABSTRACT:
The present invention discloses methods for identifying and analysing microsatellite-associated polymorphisms between different DNA samples. Different DNA samples, e.g. from different individuals, are analysed using a PCR based on the combination of a RAMP-primer and an AFLP-primer and polymorphisms between the different DNA samples are identified. The polymorphisms thus identified may be isolated and further analysed by e.g. DNA sequence analysis both upstream and downstream from the microsatellite-associated polymorphism. These sequences may subsequently be used to devise and synthesise new means for analysis of the polymorphic locus, such as e.g. PCR-primer pairs or oligonucleotide probes.

REFERENCES:
patent: 5874215 (1999-02-01), Kuiper et al.
patent: 5955276 (1999-09-01), Morgante et al.
patent: 0 534 858 (1993-03-01), None
patent: 0 721 987 (1996-07-01), None
patent: WO 98/42867 (1998-10-01), None
patent: WO 00/24935 (2000-05-01), None
Wu et al. “Detection Of Microsatellite Polymorphisms Without Cloning”Nucleic Acids Research22(15) :3257-3258 (1994).
Witsenboer et al. “Identification, Genetic Localization, And Allelic Diversity Of Selectively Amplified Microsatellite Polymorphic Loci In Lettuce And Wild Relatives (LactucaSpp.)”Genome40 (1997).
Vos et al. “AFLP: A New Technique for DNA Fingerprinting”Nucleic Acids Research23(21) :4407-4414 (1995).
Sanchez De La Hoz et al. “Simple Sequence Repeat Primers Used in Polymerase Chain Reaction Amplifications to Study Genetic Diversity in Barley”Genome39: 112-117 (1996).

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