Methods and devices for multiplexing amplification reactions

Details Methods and devices for multiplexing amplification reactions Methods and devices for multiplexing amplification reactions

2006-08-08

2006-08-08

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091200

Reexamination Certificate

active

07087414

ABSTRACT:
The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100–1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identifyE. Coliwere identified using the multiplex amplification method.

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