Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-09
1999-08-17
Witz, Jean C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435269, 435270, 536 2542, C12Q 168, C07K 100, C12N 108, C07H 2102
Patent
active
059392592
ABSTRACT:
The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, or purify nucleic acids from a treated whole blood source that has naturally occurring nucleic acid amplification inhibitors present, as well as added blood stabilization components that also inhibit nucleic acid amplification. More importantly, these nucleic acids can be released after collection or storage in a manner that enables them to be amplified by conventional techniques such as polymerase chain reaction. In particular, an absorbent material that does not bind nucleic acids irreversibly is impregnated with a chaotropic salt. A biological source sample is contacted with the impregnated absorbent material. Any nucleic acids present in the biological source can be either eluted or resolubilized off the absorbent material.
REFERENCES:
patent: 5234809 (1993-08-01), Boom et al.
patent: 5496562 (1996-03-01), Burgoyne
patent: 5501963 (1996-03-01), Burckhardt
Harvey et al. "Impregnated 903(TM) Blood Collection Oaoer: A Tool for DNA Prepartion from Dried Blood Spots for PCR Amplification", Clin. Chem. (1995) 41(6): S108.
Burghoff Robert L.
Harvey Michael A.
King Thomas H.
Kremer Richard D.
Hanley Susan
Schleicher & Schuell, Inc.
Voyce Brian D.
Witz Jean C.
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